Stem and progenitor cell division kinetics during postnatal mouse mammary gland development
Giraddi, Rajshekhar R
Blasco, Maria A
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Giraddi, R. R., Shehata, M., Gallardo, M., Blasco, M. A., Simons, B., & Stingl, J. (2015). Stem and progenitor cell division kinetics during postnatal mouse mammary gland development. Nature Communications, 6 (8487)https://doi.org/10.1038/ncomms9487
The cycling properties of mammary stem and progenitor cells is not well understood. To determine the division properties of these cells, we administered synthetic nucleosides for varying periods of time to mice at different stages of postnatal development and monitored the rate of uptake of these nucleosides in the different mammary cell compartments. Here we show that most cell division in the adult virgin gland is restricted to the oestrogen receptor-expressing luminal cell lineage. Our data also demonstrate that the oestrogen receptor-expressing, milk and basal cell subpopulations have telomere lengths and cell division kinetics that are not compatible with these cells being hierarchically organized; instead, our data indicate that in the adult homeostatic gland, each cell type is largely maintained by its own restricted progenitors. We also observe that transplantable stem cells are largely quiescent during oestrus, but are cycling during dioestrus when progesterone levels are high.
Biological sciences, Cell biology, Developmental biology
We thank the members of Stingl lab, Doug Winton, Jason Carroll, Robert Clarke, Phil Jones and Hamid Raza Ali for scientific discussions. We thank the core facilities at the Cancer Research UK-CI for enabling experiments. In particular, Loic Tauzin, Nina Lane and Mateuz Strzelecki for assistance with cell sorting; the Biological Resources Unit for animal husbandry; and Histopathology staff, in particular Leigh-Anne McDuffus and Cara Walters. J. Stingl’s laboratory acknowledges the support of The University of Cambridge, Cancer Research UK (core grant number C14303/A17197) and Hutchison Whampoa Limited. M.A. Blasco’s laboratory is funded by the Spanish Ministry of Economy and Competitiveness Project SAF2013-45111RETOS, the European Union FP7 Project EUROBATS, the European Research Council (ERC) Project TEL STEM CELL (GA#232854), the Regional Government of Madrid project 2+2 ReCaRe, the AXA Research Fund and the Fundación Botín.
Wellcome Trust (098357/Z/12/Z)
External DOI: https://doi.org/10.1038/ncomms9487
This record's URL: https://www.repository.cam.ac.uk/handle/1810/252853
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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