Uncovering the number and clonal dynamics of Mesp1 progenitors during heart morphogenesis
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Chabab, S., Lescroart, F., Rulands, S., Mathiah, N., Simons, B., & Blanpain, C. (2016). Uncovering the number and clonal dynamics of Mesp1 progenitors during heart morphogenesis. Cell Reports, 14 1-10. https://doi.org/10.1016/j.celrep.2015.12.013
The heart arises from distinct sources of cardiac progenitors that independently express Mesp1 during gastrulation. The precise number of Mesp1 progenitors that are specified during the early stage of gastrulation and their clonal behavior during heart morphogenesis is currently unknown. Here, we used clonal and mosaic tracing of Mesp1 expressing cells combined with quantitative biophysical analysis of the clonal data to define the number of cardiac progenitors and their mode of growth during heart development. Our data indicate that the myocardial layer of the heart derive from ~250 Mesp1 expressing cardiac progenitors born during gastrulation. Despite arising at different time points and contributing to different heart regions, the temporally distinct cardiac progenitors present very similar clonal dynamics. These results provide insights into the number of cardiac progenitors and their mode of growth, and opens up avenues to decipher the clonal dynamics of progenitors in other organs and tissues.
S.C. and N.M. are supported by fellowship of the FRS/FRIA. F.L has been supported by the EMBO longterm fellowship. B.D.S. and S.R. are supported by Wellcome Trust (grant number 098357/Z/12/Z). C.B. is an investigator of WELBIO. This work was supported by the FNRS, the ULB foundation, the European Research Council (ERC), and the foundation Bettencourt Schueller (C.B. and F.L.).
Wellcome Trust (098357/Z/12/Z)
External DOI: https://doi.org/10.1016/j.celrep.2015.12.013
This record's URL: https://www.repository.cam.ac.uk/handle/1810/252872
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/