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dc.contributor.authorKistler, Loganen
dc.contributor.authorNewsom, Lee Aen
dc.contributor.authorRyan, Timothy Men
dc.contributor.authorClarke, Andrewen
dc.contributor.authorSmith, Bruce Den
dc.contributor.authorPerry, George Hen
dc.date.accessioned2015-12-18T15:38:08Z
dc.date.available2015-12-18T15:38:08Z
dc.date.issued2015-11-16en
dc.identifier.citationProceedings of the National Academy of Sciences 2015, 112(49): 15107–15112. doi:10.1073/pnas.1516109112en
dc.identifier.issn0027-8424
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253045
dc.description.abstractThe genus Cucurbita (squashes, pumpkins, gourds) contains numerous domesticated lineages with ancient New World origins. It was broadly distributed in the past but has declined to the point that several of the crops’ progenitor species are scarce or unknown in the wild. We hypothesize that Holocene ecological shifts and megafaunal extinctions severely impacted wild Cucurbita, whereas their domestic counterparts adapted to changing conditions via symbiosis with human cultivators. First, we used high-throughput sequencing to analyze complete plastid genomes of 91 total Cucurbita samples, comprising ancient (n = 19), modern wild (n = 30), and modern domestic (n = 42) taxa. This analysis demonstrates independent domestication in eastern North America, evidence of a previously unknown pathway to domestication in northeastern Mexico, and broad archaeological distributions of taxa currently unknown in the wild. Further, sequence similarity between distant wild populations suggests recent fragmentation. Collectively, these results point to wild-type declines coinciding with widespread domestication. Second, we hypothesize that the disappearance of large herbivores struck a critical ecological blow against wild Cucurbita, and we take initial steps to consider this hypothesis through cross-mammal analyses of bitter taste receptor gene repertoires. Directly, megafauna consumed Cucurbita fruits and dispersed their seeds; wild Cucurbita were likely left without mutualistic dispersal partners in the Holocene because they are unpalatable to smaller surviving mammals with more bitter taste receptor genes. Indirectly, megafauna maintained mosaic-like landscapes ideal for Cucurbita, and vegetative changes following the megafaunal extinctions likely crowded out their disturbed-ground niche. Thus, anthropogenic landscapes provided favorable growth habitats and willing dispersal partners in the wake of ecological upheaval.
dc.description.sponsorshipResearch was supported by The Pennsylvania State University Huck Institutes of the Life Sciences and College of the Liberal Arts (G.H.P.), Wenner–Gren post-PhD Research Grant 8770 and Natural Environment Research Council Independent Research Fellowship NE/L012030/1 (to L.K.), and the Smithsonian Institution (B.D.S.). Instrumentation was funded by the National Science Foundation through Grant OCI–0821527.
dc.languageEnglishen
dc.language.isoenen
dc.publisherPNAS
dc.subjectevolutionary ecologyen
dc.subjectsensory ecologyen
dc.subjectTAS2R genesen
dc.subjectancient DNAen
dc.subjectarchaeogenomicsen
dc.titleGourds and squashes (Cucurbita spp.) adapted to megafaunal extinction and ecological anachronism through domesticationen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from PNAS via http://dx.doi.org/10.1073/pnas.1516109112en
prism.endingPage15112
prism.publicationDate2015en
prism.publicationNameProceedings of the National Academy of Sciencesen
prism.startingPage15107
prism.volume112en
dc.rioxxterms.funderNERC
dc.rioxxterms.projectidNE/L012030/1
rioxxterms.versionofrecord10.1073/pnas.1516109112en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-11-16en
dc.contributor.orcidClarke, Andrew [0000-0003-2293-1423]
dc.identifier.eissn1091-6490
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2016-05-16


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