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dc.contributor.authorKalantarMotamedi, Yasamanen
dc.contributor.authorPeymani, Maryamen
dc.contributor.authorBaharvand, Hosseinen
dc.contributor.authorNasr-Esfahani, Mohammad Hosseinen
dc.contributor.authorBender, Andreasen
dc.date.accessioned2016-01-13T18:31:52Z
dc.date.available2016-01-13T18:31:52Z
dc.date.issued2016-02-08en
dc.identifier.citationKalantarMotamedi et al. Cell Death Discovery (2016) Vol. 2, Article 16007. ​doi: 10.1038/cddiscovery.2016.7.en
dc.identifier.issn2058-7716
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253248
dc.description.abstractSmall molecules are being increasingly used for inducing the targeted differentiation of stem cells to different cell types. However, until now no systematic method for selecting suitable small molecules for this purpose has been presented. In this work, we propose an integrated and general bioinformatics- and cheminformatics-based approach for selecting small molecules which direct cellular differentiation in the desired way. The approach was successfully experimentally validated for differentiating stem cells into cardiomyocytes. All predicted compounds enhanced expression of cardiac progenitor (Gata4, Nkx2-5 and Mef2c) and mature cardiac markers (Actc1, myh6) significantly during and post-cardiac progenitor formation. The best-performing compound, Famotidine, increased the percentage of Myh6-positive cells from 33 to 56%, and enhanced the expression of Nkx2.5 and Tnnt2 cardiac progenitor and cardiac markers in protein level. The approach employed in the study is applicable to all other stem cell differentiation settings where gene expression data are available.
dc.description.sponsorshipYK and AB thank the European Research Council (ERC Starting Grant 2013 to AB) for funding.
dc.languageEnglishen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleSystematic selection of small molecules to promote differentiation of embryonic stem cells and experimental validation for generating cardiomyocytesen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Nature Publishing Group via http://dx.doi.org/10.1038/cddiscovery.2016.7en
prism.number16007en
prism.publicationDate2016en
prism.publicationNameCell Death Discoveryen
prism.volume2en
dc.rioxxterms.funderERC
dcterms.dateAccepted2015-12-04en
rioxxterms.versionofrecord10.1038/cddiscovery.2016.7en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-02-08en
dc.contributor.orcidBender, Andreas [0000-0002-6683-7546]
dc.identifier.eissn2058-7716
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (336159)
cam.orpheus.successThu Jan 30 10:20:17 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2300-01-01


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales