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Translocation frequency of double-stranded DNA through a solid-state nanopore.

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Peer-reviewed

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Article

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Authors

Bell, Nicholas AW 
Muthukumar, Murugappan 
Keyser, Ulrich F 

Abstract

Solid-state nanopores are single-molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage, and salt dependence of the frequency of double-stranded DNA translocations through conical quartz nanopores with mean opening diameter 15 nm. We observe an entropic barrier-limited, length-dependent translocation frequency at 4M LiCl salt concentration and a drift-dominated, length-independent translocation frequency at 1M KCl salt concentration. These observations are described by a unifying convection-diffusion equation, which includes the contribution of an entropic barrier for polymer entry.

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Keywords

Base Pairing, DNA, Movement, Nanopores, Potassium Chloride

Journal Title

Phys Rev E

Conference Name

Journal ISSN

2470-0045
2470-0053

Volume Title

93

Publisher

American Physical Society (APS)
Sponsorship
European Research Council (261101)