The chicken B-cell line DT40 proteome, beadome and interactomes.
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Authors
Rees, Johanna S
Lilley, Kathryn S
Jackson, Antony P
Abstract
In developing a new quantitative AP-MS method for exploring interactomes in the chicken B-cell line DT40, we also surveyed the most abundant proteins in this organism and explored the likely contaminants that bind to a variety of affinity resins that would later be confirmed quantitatively [1]. We present the 'Top 150 abundant DT40 proteins list', the DT40 beadomes as well as protein interaction lists for the Phosphatidyl inositol 5-phosphate 4-kinase 2β and Fanconi anaemia protein complexes.
Description
Keywords
0601 Biochemistry and Cell Biology, Biomedical
Journal Title
Data Brief
Conference Name
Journal ISSN
2352-3409
2352-3409
2352-3409
Volume Title
3
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/H024085/1)
We thanks Prof. R. Irvine for providing the JPR3 cell line, to Dr. E. Rajendra for providing the FANCC cell line, FANC antibodies and helpful discussions, to Dr. M. Deery and J. Howard for assistance with MS. This work was funded by the Biotechnology and Biological Sciences Research Council (UK) Grant BB/H024085/1.