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dc.contributor.authorda, Silva Serra Inesen
dc.contributor.authorHusson, Zoeen
dc.contributor.authorBartlett, Jonathan Den
dc.contributor.authorSmith, Ewan St Johnen
dc.date.accessioned2016-02-03T11:42:04Z
dc.date.available2016-02-03T11:42:04Z
dc.date.issued2016-03-15en
dc.identifier.citationda Silva Serra et al. Molecular Pain (2016) Vol. 12, Article 1744806916636387. doi: 10.1177/1744806916636387en
dc.identifier.issn1744-8069
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253610
dc.description.abstractBackground: A wide range of stimuli can activate sensory neurons and neurons innervating specific tissues often have distinct properties. Here we used retrograde tracing to identify sensory neurons innervating the hind paw skin (cutaneous) and ankle/knee joints (articular), and combined immunohistochemistry and electrophysiology analysis to determine the neurochemical phenotype of cutaneous and articular neurons, as well as their electrical and chemical excitability. Results: Immunohistochemistry analysis using RetroBeads as a retrograde tracer confirmed previous data that cutaneous and articular neurons are a mixture of myelinated and unmyelinated neurons, and the majority of both populations are peptidergic. In whole-cell patch-clamp recordings from cultured dorsal root ganglion neurons, voltage-gated inward currents and action potential parameters were largely similar between articular and cutaneous neurons, although cutaneous neuron action potentials had a longer half-peak duration. An assessment of chemical sensitivity showed that all neurons responded to a pH 5.0 solution, but that acid-sensing ion channel (ASIC) currents, determined by inhibition with the non-selective ASIC antagonist benzamil, were of a greater magnitude in cutaneous compared to articular neurons. 40 – 50% of cutaneous and articular neurons responded to capsaicin, cinnamaldehyde and menthol, indicating similar expression levels of TRPV1, TRPA1 and TRPM8 respectively. By contrast, significantly more articular neurons responded to ATP than cutaneous neurons. Conclusion: This work makes a detailed characterization of cutaneous and articular sensory neurons, and highlights the importance of making recordings from identified neuronal populations: sensory neurons innervating different tissues have subtly different properties, possibly reflecting different functions.
dc.description.sponsorshipISS was funded by an Erasmus for Graduate Students grant from the University of Coimbra. ZMAH and experiments were funded by an Arthritis Research Project Grant (Grant Reference 20930) to ESS. JDB was funded by a Corpus Christi College Study and Travel Grant. EStJS was funded by an Early Career Research Grant from the International Association for the Study of Pain. Thanks to Christoforos Tsantoulas for assistance with immunohistochemistry and members of the Smith lab for their technical assistance and help in preparing the manuscript.
dc.languageEnglishen
dc.language.isoenen
dc.publisherSAGE
dc.rightsAttribution-NonCommercial 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.0/uk/
dc.subjectacid-sensing ion channelen
dc.subjection channelen
dc.subjectskinen
dc.subjectjointen
dc.subjectdorsal root gangliaen
dc.subjectnociceptionen
dc.subjectpainen
dc.titleCharacterization of cutaneous and articular sensory neuronsen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from SAGE via http://dx.doi.org/10.1177/1744806916636387en
prism.number1744806916636387en
prism.publicationDate2016en
prism.publicationNameMolecular Painen
prism.volume12en
dcterms.dateAccepted2016-02-02en
rioxxterms.versionofrecord10.1177/1744806916636387en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-03-15en
dc.contributor.orcidHusson, Zoe [0000-0002-6787-1447]
dc.contributor.orcidSmith, Ewan St John [0000-0002-2699-1979]
dc.identifier.eissn1744-8069
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idInternational Association for the Study of Pain (IASP) (unknown)
pubs.funder-project-idARTHRITIS RESEARCH UK (20930)
cam.orpheus.successThu Jan 30 12:55:27 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2300-01-01


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Attribution-NonCommercial 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 2.0 UK: England & Wales