Show simple item record

dc.contributor.authorSkinner, Benjaminen
dc.contributor.authorSargent, Caroleen
dc.contributor.authorChurcher, Carolen
dc.contributor.authorHunt, Tobyen
dc.contributor.authorHerrero, Javieren
dc.contributor.authorLoveland, Jane Een
dc.contributor.authorDunn, Matten
dc.contributor.authorLouzada, Sandraen
dc.contributor.authorFu, Beiyuanen
dc.contributor.authorChow, Williamen
dc.contributor.authorGilbert, Jamesen
dc.contributor.authorAustin-Guest, Siobhanen
dc.contributor.authorBeal, Kathrynen
dc.contributor.authorCarvalho-Silva, Deniseen
dc.contributor.authorCheng, Williamen
dc.contributor.authorGordon, Dariaen
dc.contributor.authorGrafham, Darrenen
dc.contributor.authorHardy, Matten
dc.contributor.authorHarley, Joen
dc.contributor.authorHauser, Heidien
dc.contributor.authorHowden, Philipen
dc.contributor.authorHowe, Kerstinen
dc.contributor.authorLachani, Kimen
dc.contributor.authorEllis, Peter JIen
dc.contributor.authorKelly, Danielen
dc.contributor.authorKerry, Giselleen
dc.contributor.authorKerwin, Jamesen
dc.contributor.authorNg, Bee Lingen
dc.contributor.authorThreadgold, Glenen
dc.contributor.authorWileman, Thomasen
dc.contributor.authorWood, Jonathan MDen
dc.contributor.authorYang, Fengtangen
dc.contributor.authorHarrow, Jenen
dc.contributor.authorAffara, Nabeelen
dc.contributor.authorTyler-Smith, Chrisen
dc.date.accessioned2016-02-09T11:51:16Z
dc.date.available2016-02-09T11:51:16Z
dc.date.issued2015-11-11en
dc.identifier.citationSkinner et al. Genome Research (2016) Vol. 26, pp. 130-139. doi: 10.1101/gr.188839.114en
dc.identifier.issn1088-9051
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253674
dc.description.abstractWe have generated an improved assembly and gene annotation of the pig X Chromosome, and a first draft assembly of the pig Y Chromosome, by sequencing BAC and fosmid clones from Duroc animals and incorporating information from optical mapping and fiber-FISH. The X Chromosome carries 1033 annotated genes, 690 of which are protein coding. Gene order closely matches that found in primates (including humans) and carnivores (including cats and dogs), which is inferred to be ancestral. Nevertheless, several protein-coding genes present on the human X Chromosome were absent from the pig, and 38 pig-specific X-chromosomal genes were annotated, 22 of which were olfactory receptors. The pig Y-specific Chromosome sequence generated here comprises 30 megabases (Mb). A 15-Mb subset of this sequence was assembled, revealing two clusters of male-specific low copy number genes, separated by an ampliconic region including the HSFY gene family, which together make up most of the short arm. Both clusters contain palindromes with high sequence identity, presumably maintained by gene conversion. Many of the ancestral X-related genes previously reported in at least one mammalian Y Chromosome are represented either as active genes or partial sequences. This sequencing project has allowed us to identify genes—both single copy and amplified—on the pig Y Chromosome, to compare the pig X and Y Chromosomes for homologous sequences, and thereby to reveal mechanisms underlying pig X and Y Chromosome evolution.
dc.description.sponsorshipThis work was funded by BBSRC grant BB/F021372/1. The Flow Cytometry and Cytogenetics Core Facilities at the Wellcome Trust Sanger Institute and Sanger investigators are funded by the Wellcome Trust (grant number WT098051). K.B., D.C.-S., and J.H. acknowledge support from the Wellcome Trust (WT095908), the BBSRC (BB/I025506/1), and the European Molecular Biology Laboratory. The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 222664 (“Quantomics”).
dc.languageEnglishen
dc.language.isoenen
dc.publisherCold Spring Harbor Laboratory Press
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.titleThe pig X and Y Chromosomes: structure, sequence, and evolutionen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Cold Spring Harbor Laboratory Press via http://dx.doi.org/10.1101/gr.188839.114en
prism.endingPage139
prism.publicationDate2015en
prism.publicationNameGenome Researchen
prism.startingPage130
dc.rioxxterms.funderBBSRC
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderBB/F021372/1
dc.rioxxterms.funderBB/I025506/1
dc.rioxxterms.projectidWT098051
dc.rioxxterms.projectidWT095908
dcterms.dateAccepted2015-11-09en
rioxxterms.versionofrecord10.1101/gr.188839.114en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-11-11en
dc.contributor.orcidSkinner, Benjamin [0000-0002-7152-1167]
dc.contributor.orcidSargent, Carole [0000-0002-4205-3085]
dc.identifier.eissn1549-5469
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/F021372/1)


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales