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dc.contributor.authorWan, Yinan
dc.contributor.authorAlmeida, Alexandra D
dc.contributor.authorRulands, Steffen
dc.contributor.authorChalour, Naima
dc.contributor.authorMuresan, Leila
dc.contributor.authorWu, Yunmin
dc.contributor.authorSimons, Benjamin D
dc.contributor.authorHe, Jie
dc.contributor.authorHarris, William A
dc.date.accessioned2016-02-15T16:31:15Z
dc.date.available2016-02-15T16:31:15Z
dc.date.issued2016-04-01
dc.identifier.citationWan et al. Development (2016)
dc.identifier.issn0950-1991
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253761
dc.description.abstractClonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues (Simons and Clevers, 2011). Such an analysis, however, has not yet been achieved for continuously growing adult tissues, but is essential if we wish to understand the architecture of adult organs. The retinas of lower vertebrates grow throughout life from retinal stem cells (RSCs) and retinal progenitor cells (RPCs) at the rim of the retina, called the ciliary marginal zone (CMZ). Here, we show that RSCs reside in a niche at the extreme periphery of the CMZ and divide asymmetrically along a radial (peripheral to central) axis, leaving one daughter in the peripheral RSC niche and the other more central where it becomes an RPC. We also show that RPCs of the CMZ have clonal sizes and compositions that are statistically similar to progenitor cells of the embryonic retina and fit the same stochastic model of proliferation. These results link embryonic and postembryonic cell behaviour, and help to explain the constancy of tissue architecture that has been generated over a lifetime.
dc.description.sponsorshipThis work was supported by a Wellcome Trust Senior Investigator Awards [100329/Z/12/Z to W.A.H.] and [098357/Z/12/Z to B.D.S].
dc.languageEnglish
dc.language.isoen
dc.publisherThe Company of Biologists
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectstem cells
dc.subjectprogenitor cells
dc.subjectretina
dc.subjectciliary marginal zone
dc.subjectlive imaging
dc.subjectclonal analysis
dc.subjectzebrafish
dc.titleThe ciliary marginal zone of the zebrafish retina: clonal and time-lapse analysis of a continuously growing tissue.
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from The Company of Biologists via https://doi.org/10.1242/dev.133314
prism.endingPage1107
prism.publicationDate2016
prism.publicationNameDevelopment
prism.startingPage1099
prism.volume143
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.projectid100329/Z/12/Z
dc.rioxxterms.projectid098357/Z/12/Z
dcterms.dateAccepted2016-02-09
rioxxterms.versionofrecord10.1242/dev.133314
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2016-02-18
dc.contributor.orcidRulands, Steffen [0000-0001-6398-1553]
dc.contributor.orcidMuresan, Leila [0000-0002-7602-0249]
dc.contributor.orcidSimons, Benjamin [0000-0002-3875-7071]
dc.contributor.orcidHarris, William [0000-0002-9995-8096]
dc.identifier.eissn1477-9129
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (100329/Z/12/Z)
pubs.funder-project-idWellcome Trust (098357/Z/12/Z)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
cam.issuedOnline2016-02-18
cam.orpheus.successThu Jan 30 12:55:10 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International