SLTAB2 is the paramutated SULFUREA locus in tomato
Journal of Experimental Botany
Oxford University Press
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Gouil, Q., Novák, O., & Baulcombe, D. (2016). SLTAB2 is the paramutated SULFUREA locus in tomato. Journal of Experimental Botany, 67 2655-2664. https://doi.org/10.1093/jxb/erw096
The sulfurea (sulf) allele is a silent epigenetic variant of a tomato (Solanum lycopersicum) gene affecting pigment production. It is homozygous lethal but, in a heterozygote sulf/+, the wild-type (wt) allele undergoes silencing so that the plants exhibit chlorotic sectors. This transfer of the silenced state between alleles is termed paramutation and is best characterized in maize. To understand the mechanism of paramutation we mapped SULF to the orthologue SLTAB2 of an Arabidopsis gene that, consistent with the pigment deﬁciency, is involved in the translation of photosystem I. Paramutation of SLTAB2 is linked to an increase in DNA methylation and the production of small interfering RNAs at its promoter. Virus-induced gene silencing of SLTAB2 phenocopies sulf, consistent with the possibility that siRNAs mediate the paramutation of SULFUREA. Unlike the maize systems, the paramutagenicity of sulf is not, however, associated with repeated sequences at the region of siRNA production or DNA methylation.
Auxin, DNA methylation, paramutation, Photosynthesis, RdDM, siRNA, sulfurea, VIGS
This work was supported by the European Research Council Advanced Investigator Grant ERC-2013-AdG 340642, the Frank Smart Studentship and the Ministry of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability I Nr. LO1204). D.C.B. is the Royal Society Edward Penley Abraham Research Professor.
European Research Council (340642)
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External DOI: https://doi.org/10.1093/jxb/erw096
This record's URL: https://www.repository.cam.ac.uk/handle/1810/253831