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dc.contributor.authorSchütte, Judithen
dc.contributor.authorWang, Huangeen
dc.contributor.authorAntoniou, Stellaen
dc.contributor.authorJarratt, Andrewen
dc.contributor.authorWilson, Nicolaen
dc.contributor.authorRiepsaame, Joeyen
dc.contributor.authorCalero-Nieto, Fernando Jen
dc.contributor.authorMoignard, Victoriaen
dc.contributor.authorBasilico, Silviaen
dc.contributor.authorKinston, Sarahen
dc.contributor.authorHannah, Rebeccaen
dc.contributor.authorChan, Mun Chiangen
dc.contributor.authorNürnberg, Sylvia Ten
dc.contributor.authorOuwehand, Willemen
dc.contributor.authorBonzanni, Nicolaen
dc.contributor.authorde, Bruijn Marella FTRen
dc.contributor.authorGottgens, Bertholden
dc.date.accessioned2016-02-22T14:48:24Z
dc.date.available2016-02-22T14:48:24Z
dc.date.issued2016-02-22en
dc.identifier.citationSchütte et al. eLife (2016). doi: 10.7554/eLife.11469en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253879
dc.description.abstractTranscription factor (TF) networks determine cell type identity by establishing and maintaining lineage-specific expression profiles, yet reconstruction of mammalian regulatory network models has been hampered by a lack of comprehensive functional validation of regulatory interactions. Here, we report comprehensive ChIP-Seq, transgenic and reporter gene experimental data that have allowed us to construct an experimentally validated regulatory network model for haematopoietic stem/progenitor cells (HSPCs). Model simulation coupled with subsequent experimental validation using single cell expression profiling revealed potential mechanisms for cell state stabilisation, and also how a leukemogenic TF fusion protein perturbs key HSPC regulators. The approach presented here should help to improve our understanding of both normal physiological and disease processes.
dc.description.sponsorshipResearch in the authors’ laboratories was supported by Bloodwise, The Wellcome Trust, Cancer Research UK, the Biotechnology and Biological Sciences Research Council, the National Institute of Health Research, the Medical Research Council, the MRC Molecular Haematology Unit (Oxford) core award, a Weizmann-UK “Making Connections” grant (Oxford) and core support grants by the Wellcome Trust to the Cambridge Institute for Medical Research (100140) and Wellcome Trust–MRC Cambridge Stem Cell Institute (097922).
dc.languageEnglishen
dc.language.isoenen
dc.publishereLife
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.titleAn experimentally validated network of nine haematopoietic transcription factors reveals mechanisms of cell state stabilityen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from eLife via http://dx.doi.org/10.7554/eLife.11469en
prism.numbere11469en
prism.publicationDate2016en
prism.publicationNameeLifeen
prism.volume5en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderCRUK
dc.rioxxterms.funderBBSRC
dc.rioxxterms.funderNIHR
dc.rioxxterms.funderMRC
dcterms.dateAccepted2016-02-12en
rioxxterms.versionofrecord10.7554/eLife.11469en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-02-22en
dc.contributor.orcidWilson, Nicola [0000-0003-0865-7333]
dc.contributor.orcidMoignard, Victoria [0000-0001-5089-5875]
dc.contributor.orcidKinston, Sarah [0000-0002-5217-098X]
dc.contributor.orcidHannah, Rebecca [0000-0003-0477-7792]
dc.contributor.orcidOuwehand, Willem [0000-0002-7744-1790]
dc.contributor.orcidGottgens, Berthold [0000-0001-6302-5705]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (12765)
pubs.funder-project-idLeukaemia & Lymphoma Research (12029)
pubs.funder-project-idBBSRC (BB/I00050X/1)
pubs.funder-project-idCancer Research UK (CRUK-A19405)
pubs.funder-project-idWellcome Trust (097922/Z/11/Z)
pubs.funder-project-idMRC (MC_PC_12009)
pubs.funder-project-idLeukemia & Lymphoma Society (7001-12)
pubs.funder-project-idMEDICAL RESEARCH COUNCIL (MR/M008975/1)
pubs.funder-project-idMRC (G0900951)
pubs.funder-project-idWellcome Trust (100140/Z/12/Z)
pubs.funder-project-idWellcome Trust (097922/Z/11/B)
pubs.funder-project-idBritish Heart Foundation (RG/09/012/28096)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales