Selective, non-covalent conjugation of synthetic peptides with recombinant proteins mediated by host-guest chemistry.
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Article
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Abstract
The combination of potent chemical moieties with biologically active proteins is key to some of today's most innovative therapeutic drugs. In order to obviate any chemical modification of the proteins, we present a novel and powerful strategy for the selective conjugation of recombinant protein domains with synthetically derived peptides via a cucurbit[8]uril host-guest chemistry approach.
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Keywords
Peptides, Recombinant Proteins
Journal Title
Chem Commun (Camb)
Conference Name
Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
52
Publisher
Royal Society of Chemistry (RSC)
Publisher DOI
Sponsorship
European Research Council (240629)
We thank Alexandra Rieck, Isabelle Sermadiras, Benjamin Kemp and Jennifer Spooner for help with the expression and purification of the Fc and Tn3 domains, Jefferson Revell and Jacky Metcalfe for help with the synthesis of modified peptides and Christel Veyssier for help with the light scattering experiments. RJG was supported by the MedImmune postdoctoral program. SS was supported by ERC Starting Investigator grant ASPiRe (No. 240629).