The RNA Helicase eIF4A is required for Sapovirus translation
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Peer-reviewed
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Abstract
The eukaryotic initiation factor (eIF) 4A is a DEAD-box helicase that unwinds RNA structure in the 5´-untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5´-UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
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Keywords
5' Untranslated Regions, Animals, Eukaryotic Initiation Factor-4A, Genome, Viral, Mutation, Protein Binding, RNA, Viral, Rabbits, Reticulocytes, Sapovirus, Sterols, Swine, Viral Proteins, Virus Replication
Journal Title
Journal of Virology
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0022-538X
1098-5514
1098-5514
Volume Title
90
Publisher
American Society for Microbiology
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Wellcome Trust (097997/Z/11/Z)
Biotechnology and Biological Sciences Research Council (BB/I012303/2)
Biotechnology and Biological Sciences Research Council (BB/N001176/1)
Biotechnology and Biological Sciences Research Council (BB/I012303/2)
Biotechnology and Biological Sciences Research Council (BB/N001176/1)
This work was supported by Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (2014R1A2A2A01004292), and by funding to IG (Wellcome Senior Fellow) from the Wellcome Trust (Ref: 097997/Z/11/Z) and Biological and Biotechnology Research Council (BBSRC) (Ref: BB/I012303/1) and by BBSRC funding to SC (Ref: BB/J001708/1). TS is a Marie Curie Fellow (Ref:628373). We thank Professor Jerry Pelletier (McGill University, Canada) for providing hippuristanol.