Show simple item record

dc.contributor.authorHosmilloa, Myraen
dc.contributor.authorSweeney, Trevoren
dc.contributor.authorChaudhry, Yasminen
dc.contributor.authorLeenc, Eoinen
dc.contributor.authorCurry, Stephenen
dc.contributor.authorGoodfellow, Ianen
dc.contributor.authorChoa, Kyoung-Ohen
dc.date.accessioned2016-03-03T10:19:38Z
dc.date.available2016-03-03T10:19:38Z
dc.identifier.citationHosmillo et al. Journal of Virology (2016)en
dc.identifier.issn0022-538X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/254121
dc.description.abstractThe eukaryotic initiation factor (eIF) 4A is a DEAD-box helicase that unwinds RNA structure in the 5´-untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5´-UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
dc.description.sponsorshipThis work was supported by Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (2014R1A2A2A01004292), and by funding to IG (Wellcome Senior Fellow) from the Wellcome Trust (Ref: 097997/Z/11/Z) and Biological and Biotechnology Research Council (BBSRC) (Ref: BB/I012303/1) and by BBSRC funding to SC (Ref: BB/J001708/1). TS is a Marie Curie Fellow (Ref:628373). We thank Professor Jerry Pelletier (McGill University, Canada) for providing hippuristanol.
dc.languageEnglishen
dc.language.isoenen
dc.publisherAmerican Society for Microbiology
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe RNA Helicase eIF4A is required for Sapovirus translationen
dc.typeArticle
prism.endingPage5204
prism.publicationNameJournal of Virologyen
prism.startingPage5200
prism.volume90en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectid097997/Z/11/Z
dc.rioxxterms.projectidBB/I012303/1
dc.rioxxterms.projectidBB/J001708/1
pubs.volume90
dcterms.dateAccepted2016-02-26en
rioxxterms.versionofrecord10.1128/JVI.03174-15en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-02-26en
dc.contributor.orcidGoodfellow, Ian [0000-0002-9483-510X]
dc.identifier.eissn1098-5514
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (097997/Z/11/Z)
pubs.funder-project-idBBSRC (BB/I012303/2)
pubs.funder-project-idBBSRC (BB/N001176/1)
cam.issuedOnline2016-04-29en
cam.orpheus.successThu Jan 30 12:55:02 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International