Detection of colorectal dysplasia using fluorescently labelled lectins
Authors
Kuo, Joe Chin-Hun
Ibrahim, Ashraf EK
Parashar, Deepak
Howat, William J
Guttula, Kiran
Miller, Richard
Fearnhead, Nicola S
Winton, Douglas
Publication Date
2016-04-13Journal Title
Scientific Reports
ISSN
2045-2322
Publisher
Nature Publishing Group
Volume
6
Number
24231
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Kuo, J. C., Ibrahim, A. E., Dawson, S., Parashar, D., Howat, W. J., Guttula, K., Miller, R., et al. (2016). Detection of colorectal dysplasia using fluorescently labelled lectins. Scientific Reports, 6 (24231)https://doi.org/10.1038/srep24231
Abstract
Colorectal cancer screening using conventional colonoscopy lacks molecular information and can miss dysplastic lesions. We tested here the ability of fluorescently labelled lectins to distinguish dysplasia from normal tissue when sprayed on to the luminal surface epithelium of freshly resected colon tissue from the Apc^min mouse and when applied to fixed human colorectal tissue sections. Wheat germ agglutinin (WGA) showed significantly decreased binding to adenomas in the mouse tissue and in sections of human colon from 47 patients. Changes in WGA binding to the human surface epithelium allowed regions containing normal epithelium (NE) or hyperplastic polyps (HP) to be distinguished from regions containing low-grade dysplasia (LGD), high-grade dysplasia (HGD) or carcinoma (C), with 81% sensitivity, 87% specificity and 93% positive predictive value (PPV). Helix pomatia agglutinin (HGA) distinguished epithelial regions containing NE from regions containing HP, LGD, HGD or C, with 89% sensitivity, 87% specificity and 97% PPV. The decreased binding of WGA and HPA to the luminal surface epithelium in human dysplasia suggests that these lectins may enable more sensitive detection of disease in the clinic using fluorescence colonoscopy.
Keywords
cancer imaging, imaging techniques and agents, translational research, early detection, colon cancer
Sponsorship
This work was supported by grants from Cancer Research UK (17242, 16465) to KMB.
Funder references
Cancer Research UK (C14303_do not transfer)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1038/srep24231
This record's URL: https://www.repository.cam.ac.uk/handle/1810/254756
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International