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dc.contributor.authorBell, Nicholasen
dc.contributor.authorKeyser, Ulrichen
dc.date.accessioned2016-04-05T15:11:36Z
dc.date.available2016-04-05T15:11:36Z
dc.date.issued2016-04-04en
dc.identifier.citationBell & Keyser. Nature Nanotechnology (2016). doi: 10.1038/nnano.2016.50en
dc.identifier.issn1748-3387
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/254812
dc.description.abstractThe simultaneous detection of a large number of different analytes is important in bionanotechnology research and in diagnostic applications. Nanopore sensing is an attractive method in this regard as the approach can be integrated into small, portable device architectures, and there is significant potential for detecting multiple sub-populations in a sample. Here, we show that highly multiplexed sensing of single molecules can be achieved with solid-state nanopores by using digitally encoded DNA nanostructures. Based on the principles of DNA origami, we designed a library of DNA nanostructures in which each member contains a unique barcode; each bit in the barcode is signalled by the presence or absence of multiple DNA dumbbell hairpins. We show that a 3-bit barcode can be assigned with 94% accuracy by electrophoretically driving the DNA structures through a solid-state nanopore. Select members of the library were then functionalized to detect a single, specific antibody through antigen presentation at designed positions on the DNA. This allows us to simultaneously detect four different antibodies of the same isotype at nanomolar concentration levels.
dc.description.sponsorshipN.A.W.B. and U.F.K. acknowledge funding from an ERC starting grant (Passmembrane 261101) and an ERC consolidator grant (Designerpores 647144). N.A.W.B. also acknowledges funding from an EPSRC doctoral prize award.
dc.languageEnglishen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.subjectDNA nanotechnologyen
dc.subjectnanoporesen
dc.titleDigitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanoporesen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from Nature Publishing Group via https://doi.org/10.1038/nnano.2016.50en
prism.endingPage651
prism.publicationDate2016en
prism.publicationNameNature Nanotechnologyen
prism.startingPage645
prism.volume11en
dc.rioxxterms.funderEPSRC
dcterms.dateAccepted2016-02-26en
rioxxterms.versionofrecord10.1038/nnano.2016.50en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-04-04en
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.identifier.eissn1748-3395
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
pubs.funder-project-idEuropean Research Council (261101)
rioxxterms.freetoread.startdate2016-10-04


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