mCSM-AB: a web server for predicting antibody-antigen affinity changes upon mutation with graph-based signatures.
Published version
Peer-reviewed
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Repository DOI
Change log
Authors
Pires, Douglas EV
Ascher, David B
Abstract
Computational methods have traditionally struggled to predict the effect of mutations in antibody-antigen complexes on binding affinity. This has limited their usefulness during antibody engineering and development, and their ability to predict biologically relevant escape mutations. Here we present mCSM-AB, a user-friendly web server for accurately predicting antibody-antigen affinity changes upon mutation which relies on graph-based signatures. We show that mCSM-AB performs better than comparable methods that have been previously used for antibody engineering. mCSM-AB web server is available at http://structure.bioc.cam.ac.uk/mcsm_ab.
Description
Keywords
Antibodies, Antibody Affinity, Antigen-Antibody Complex, Antigens, Benchmarking, Datasets as Topic, HIV Antibodies, Internet, Mutation, Protein Engineering, Software, Vaccines
Journal Title
Nucleic Acids Res
Conference Name
Journal ISSN
0305-1048
1362-4962
1362-4962
Volume Title
Publisher
Oxford University Press (OUP)
Publisher DOI
Sponsorship
Medical Research Council (MR/M026302/1)