Pins is not Required for Spindle Orientation in the $\textit{Drosophila}$ Wing Disc
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Authors
Lovegrove, Holly E
Kujawiak, Izabela
Dawney, Nicole S
Zhu, Jinwei
Cooper, Samantha
Zhang, Rongguang
Publication Date
2016-06-10Journal Title
Development
ISSN
0950-1991
Publisher
The Company of Biologists
Language
English
Type
Article
This Version
AM
Metadata
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Bergstralh, D., Lovegrove, H. E., Kujawiak, I., Dawney, N. S., Zhu, J., Cooper, S., Zhang, R., & et al. (2016). Pins is not Required for Spindle Orientation in the $\textit{Drosophila}$ Wing Disc. Development https://doi.org/10.1242/dev.135475
Abstract
In animal cells, mitotic spindles are oriented by the dynein/dynactin motor complex, which exerts a pulling force on astral microtubules. Dynein/dynactin localization depends on Mud/NUMA, which is typically recruited to the cortex by Pins/LGN. In $\textit{Drosophila}$ neuroblasts, the Inscuteable/Baz/Par-6/aPKC complex recruits Pins apically to induce vertical spindle orientation, whereas in epithelial cells, Dlg recruits Pins laterally to orient the spindle horizontally. Here we investigate division orientation in the $\textit{Drosophila}$ imaginal wing disc epithelium. Live imaging reveals that spindle angles vary widely during prometaphase and metaphase, and therefore do not reliably predict division orientation. This finding prompted us to re-examine mutants that have been reported to disrupt division orientation in this tissue. Loss of Mud/NUMA misorients divisions, but Inscuteable expression and $\textit{aPKC}$, $\textit{dlg}$ and $\textit{pins}$ mutants have no effect. Furthermore, Mud localizes to the apical-lateral cortex of the wing epithelium independently of both Pins and cell cycle stage. Thus, Pins is not required in the wing disc because there are parallel mechanisms for Mud localization and hence spindle orientation, making it a more robust system than other epithelia.
Keywords
spindle orientation, Drosophila, Mud/NuMA, Pins/LGN, epithelia
Sponsorship
This work was supported by a Wellcome Trust Principal Fellowship to DStJ [080007] and by core support from the Wellcome Trust [092096] and Cancer Research UK [A14492]. DTB was supported by a Marie Curie Fellowship and the Wellcome Trust. HEL was supported by a Herchel Smith Studentship.
Funder references
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (080007/Z/06/Z)
Cancer Research UK (A14492)
Identifiers
External DOI: https://doi.org/10.1242/dev.135475
This record's URL: https://www.repository.cam.ac.uk/handle/1810/256538
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International
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