Research data supporting "Single cell studies of mouse embryonic stem cell (mESC) differentiation by electrical impedance measurements in a microfluidic device"
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Authors
Zhou, Ying
Basu, Srinjan
Laue, Ernest
Seshia, Ashwin A.
Description
Electrical impedance measurements for mouse embryonic stem cells (mESCs) were performed to study the embryonic stem cell differentiation process. The electrical frequency response (e.g. impedance magnitude and phase) of individual stem cells during the course of differentiation has been studied. Electrical parameters (e.g. permittivity and conductivity of cell membrane, cytoplasm, nuclear membrane, nucleoplasm, etc.) of stem cells at different differentiation states have been extracted and quantitatively analysed.
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Origin, OriginPro, and Origin Viewer can be used to read .opj files. Matlab can be used to open .m and .fig files.
Keywords
microfluidics, single cell trapping, impedance spectroscopy, stem cells, heterogeneity
Publisher
University of Cambridge
Sponsorship
BBSRC [BB/K013726/1]