Research data supporting "A microfluidic platform for trapping, releasing and super-resolution imaging of single cells"
Wohlfahrt, Kai J.
Lee, Steven F.
Laue, Ernest D.
Seshia, Ashwin A.
University of Cambridge
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Zhou, Y., Basu, S., Wohlfahrt, K. J., Lee, S. F., Klenerman, D., Laue, E. D., & Seshia, A. A. (2016). Research data supporting "A microfluidic platform for trapping, releasing and super-resolution imaging of single cells" [Dataset]. https://doi.org/10.17863/CAM.589
Particles and cells were trapped and released using a microfluidic device. The device enabled the trapping of single particles with a trapping efficiency of greater than 95%, and allowed for single particles and cells to be trapped, released and manipulated by simply controlling corresponding valves. Stable immobilisation of large numbers of single cells could be achieved in a few minutes. Proof-of-concept super-resolution imaging experiments with mouse embryonic stem cells (mESCs) were conducted by exploiting super-resolution photoactivated localisation microscopy (PALM). Cells and nuclei were stably trapped and imaged. Centromeres of ∼200 nm size could be identified with a localisation precision of <15 nm.
hydrodynamic trapping, particle manipulation, single cell analysis, super-resolution imaging, embryonic stem cells
Publication Reference: https://doi.org/10.1016/j.snb.2016.03.131
This record's DOI: https://doi.org/10.17863/CAM.589
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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