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Probing amyloid protein aggregation with optical superresolution methods: from the test tube to models of disease.

Published version
Peer-reviewed

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Authors

Kaminski, Clemens F 
Kaminski Schierle, Gabriele S 

Abstract

The misfolding and self-assembly of intrinsically disordered proteins into insoluble amyloid structures are central to many neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Optical imaging of this self-assembly process in vitro and in cells is revolutionizing our understanding of the molecular mechanisms behind these devastating conditions. In contrast to conventional biophysical methods, optical imaging and, in particular, optical superresolution imaging, permits the dynamic investigation of the molecular self-assembly process in vitro and in cells, at molecular-level resolution. In this article, current state-of-the-art imaging methods are reviewed and discussed in the context of research into neurodegeneration.

Description

Keywords

Alzheimer’s disease, protein aggregation, protein misfolding, superresolution microscopy

Journal Title

Neurophotonics

Conference Name

Journal ISSN

2329-423X
2329-4248

Volume Title

3

Publisher

SPIE-Intl Soc Optical Eng
Sponsorship
Medical Research Council (MR/N012453/1)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Medical Research Council (G0902243)
Medical Research Council (MC_G1000734)
Medical Research Council (MR/K015850/1)
Medical Research Council (MR/K02292X/1)
This work was funded by grants from the Wellcome Trust, the Medical Research Council UK, the Alzheimer Research UK Trust, the Engineering and Physical Sciences Research Council UK, the Biotechnology and Biological Sciences Research Council, and the Swiss National Science Foundation.