Multiple Bcl-2 family immunomodulators from vaccinia virus regulate MAPK/AP-1 activation
Torres, Alice A
Albarnaz, Jonas D
Bonjardim, Cláudio A
Journal of General Virology
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Torres, A. A., Albarnaz, J. D., Bonjardim, C. A., & Smith, G. (2016). Multiple Bcl-2 family immunomodulators from vaccinia virus regulate MAPK/AP-1 activation. Journal of General Virology https://doi.org/10.1099/jgv.0.000525
Vaccinia virus (VACV) is a poxvirus and encodes many proteins that modify the host cell metabolism or inhibit the host response to infection. For instance, it is known that VACV infection can activate the MAPK/AP-1 pathway and inhibit activation of the pro-inflammatory transcription factor NF-κB. Since NF-κB and MAPK/AP-1 share common upstream activators we investigated whether six different VACV Bcl-2-like NF-κB inhibitors can also influence MAPK/AP-1 activation. Data presented show that proteins A52, B14 and K7 each contribute to AP-1 activation during VACV infection and when expressed individually outwith infection. B14 induced the greatest stimulation of AP-1 and further investigation showed B14 activated mainly the MAPKs ERK and JNK, and their substrate c-Jun (a component of AP-1). These data indicate that the same viral protein can have different effects on distinct signalling pathways, in blocking NF-κB activation whilst leading to MAPK/AP-1 activation.
This work was funded by grants from the Medical Research Council, the Wellcome Trust, the Minas Gerais State’s Foundation for Research Support (FAPEMIG) and the National Council for Scientific and Technological Development (CNPq - Brazil).
Wellcome Trust (090315/B/09/Z)
WELLCOME TRUST (090315/B/09/A)
External DOI: https://doi.org/10.1099/jgv.0.000525
This record's URL: https://www.repository.cam.ac.uk/handle/1810/256755