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dc.contributor.authorNisbet, Ellenen
dc.contributor.authorKurniawan, Davy Pen
dc.contributor.authorBowers, Harrison Den
dc.contributor.authorHowe, Christopheren
dc.date.accessioned2016-08-03T07:29:09Z
dc.date.available2016-08-03T07:29:09Z
dc.date.issued2016-06-29en
dc.identifier.issn1434-4610
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/256953
dc.description.abstractThe apicoplast, an organelle found in $\textit{Plasmodium}$ and many other parasitic apicomplexan species, is a remnant chloroplast that is no longer able to carry out photosynthesis. Very little is known about primary transcripts and RNA processing in the $\textit{Plasmodium}$ apicoplast, although processing in chloroplasts of some related organisms (chromerids and dinoflagellate algae) shows a number of unusual features, including RNA editing and the addition of 3$^\prime$ poly(U) tails. Here, we show that many apicoplast transcripts are polycistronic and that there is extensive RNA processing, often involving the excision of tRNA molecules. We have identified major RNA processing sites, and have shown that these are associated with a conserved sequence motif. We provide the first evidence for the presence of RNA editing in the $\textit{Plasmodium}$ apicoplast, which has evolved independently from editing in dinoflagellates. We also present evidence for long, polycistronic antisense transcripts, and show that in some cases these are processed at the same sites as sense transcripts. Together, this research has significantly enhanced our understanding of the evolution of chloroplast RNA processing in the Apicomplexa and dinoflagellate algae.
dc.description.sponsorshipWellcome Trust (Grant ID: 094249)
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectapicoplasten
dc.subjectchloroplasten
dc.subjectRNA processingen
dc.subject$\textit{Plasmodium}$en
dc.subjectantisense RNAen
dc.subjectRNA editingen
dc.titleTranscripts in the $\textit{Plasmodium}$ Apicoplast Undergo Cleavage at tRNAs and Editing, and Include Antisense Sequencesen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Elsevier via http://dx.doi.org/10.1016/j.protis.2016.06.003en
prism.endingPage388
prism.publicationDate2016en
prism.publicationNameProtisten
prism.startingPage377
prism.volume167en
dc.identifier.doi10.17863/CAM.886
dcterms.dateAccepted2016-06-23en
rioxxterms.versionofrecord10.1016/j.protis.2016.06.003en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-06-29en
dc.contributor.orcidNisbet, Ellen [0000-0003-4487-196X]
dc.contributor.orcidHowe, Christopher [0000-0002-6975-8640]
dc.identifier.eissn1618-0941
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMEDICAL RESEARCH COUNCIL (MC_PC_15042)
pubs.funder-project-idWellcome Trust (094249/Z/10/Z)
cam.orpheus.successThu Jan 30 12:57:22 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International