A +1 ribosomal frameshifting motif prevalent among plant amalgaviruses
Nibert, Max L
Pyle, Jesse D
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Nibert, M. L., Pyle, J. D., & Firth, A. (2016). A +1 ribosomal frameshifting motif prevalent among plant amalgaviruses. Virology, 498 201-208. https://doi.org/10.1016/j.virol.2016.07.002
Multiple sequence accessions attributable to novel plant amalgaviruses have been found in the Transcriptome Shotgun Assembly database. Sixteen accessions, derived from 12 different plant species, appear to encompass the complete protein-coding regions of the proposed amalgaviruses, which would substantially expand the size of genus Amalgavirus from 4 current species. Other findings include evidence for UUU_CGN as a +1 ribosomal frameshifting motif prevalent among plant amalgaviruses; for a variant version of this motif found thus far in only two amalgaviruses from solanaceous plants; for a region of α-helical coiled coil propensity conserved in a central region of the ORF1 translation product of plant amalgaviruses; and for conserved sequences in a C-terminal region of the ORF2 translation product (RNA-dependent RNA polymerase) of plant amalgaviruses, beyond the region of conserved polymerase motifs. These results additionally illustrate the value of mining the TSA database and others for novel viral sequences for comparative analyses.
Amalgaviridae, coiled coil, database mining, dsRNA virus, fungal virus, plant virus, ribosomal frameshifting
M.L.N. was supported in part by a subcontract from NIH grant 5R01GM033050-33. J.D.P. completed his work on this project during a lab rotation for the Ph.D. Training Program in Virology at Harvard University, Cambridge, MA, USA and was supported in part by NIH grant 2T32AI007245-31. A.E.F. was supported in part by the Wellcome Trust (grant 106207).
Wellcome Trust (106207/Z/14/Z)
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External DOI: https://doi.org/10.1016/j.virol.2016.07.002
This record's URL: https://www.repository.cam.ac.uk/handle/1810/257259
Attribution-NonCommercial-NoDerivatives 4.0 International, Attribution-NonCommercial-NoDerivatives 4.0 International, Attribution-NonCommercial-NoDerivatives 4.0 International
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