Contributions of Mammalian Chimeras to Pluripotent Stem Cell Research.
Published version
Peer-reviewed
Repository URI
Repository DOI
Type
Article
Change log
Authors
Mascetti, Victoria L
Pedersen, Roger A
Abstract
Chimeras are widely acknowledged as the gold standard for assessing stem cell pluripotency, based on their capacity to test donor cell lineage potential in the context of an organized, normally developing tissue. Experimental chimeras provide key insights into mammalian developmental mechanisms and offer a resource for interrogating the fate potential of various pluripotent stem cell states. We highlight the applications and current limitations presented by intra- and inter-species chimeras and consider their future contribution to the stem cell field. Despite the technical and ethical demands of experimental chimeras, including human-interspecies chimeras, they are a provocative resource for achieving regenerative medicine goals.
Description
Keywords
Animals, Cell Lineage, Chimera, Embryo, Mammalian, Humans, Pluripotent Stem Cells, Species Specificity, Stem Cell Research
Journal Title
Cell Stem Cell
Conference Name
Journal ISSN
1934-5909
1875-9777
1875-9777
Volume Title
19
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Medical Research Council (MC_PC_12009)
British Heart Foundation Centre of Regenerative Medicine, Wellcome Trust, Medical Research Council Cambridge Stem Cell Institute, Cambridge NIHR Biomedical Research Centre