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Flow analysis of the low Reynolds number swimmer C. elegans

Accepted version
Peer-reviewed

Type

Article

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Authors

Montenegro-Johnson, Thomas D 
Gagnon, David A 
Arratia, Paulo E 

Abstract

Swimming cells and microorganisms are a critical component of many biological processes. In order to better interpret experimental studies of low Reynolds number swimming, we combine experimental and numerical methods to perform an analysis of the flow field around the swimming nematode Caenorhabditis elegans. We first use image processing and particle tracking velocimetry to extract the body shape, kinematics, and flow fields around the nematode. We then construct a three-dimensional model using the experimental swimming kinematics and employ a boundary element method to simulate flow fields, obtaining very good quantitative agreement with experiment. We use this numerical model to show that calculation of flow shear rates using purely planar data results in significant underestimates of the true three-dimensional value. Applying symmetry arguments, validated against numerics, we demonstrate that the out-of-plane contribution can be accounted for via incompressibility and therefore simply calculated from particle tracking velocimetry. Our results show how fundamental fluid mechanics considerations may be used to improve the accuracy of measurements in biofluiddynamics.

Description

Keywords

4012 Fluid Mechanics and Thermal Engineering, 40 Engineering

Journal Title

PHYSICAL REVIEW FLUIDS

Conference Name

Journal ISSN

2469-990X
2469-990X

Volume Title

1

Publisher

American Physical Society (APS)
Sponsorship
T.D.M.-J. is supported by a Royal Commission for the Exhibition of 1851 Research Fellowship and D.A.G. is supported by an NSF Graduate Fellowship. Funding from the European Union (CIG Grant to E.L.) and NSF-CBET-1437482 (to P.E.A.) is gratefully acknowledged.