Odd-paired controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory network
View / Open Files
Publication Date
2016-08-15Journal Title
eLife
ISSN
2050-084X
Publisher
eLife Sciences Publications Ltd
Volume
5
Number
e18215
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Clark, E., & Akam, M. (2016). Odd-paired controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory network. eLife, 5 (e18215)https://doi.org/10.7554/eLife.18215
Abstract
The Drosophila embryo transiently exhibits a double-segment periodicity, defined by the expression of seven 'pair-rule' genes, each in a pattern of seven stripes. At gastrulation, interactions between the pair-rule genes lead to frequency doubling and the patterning of 14 parasegment boundaries. In contrast to earlier stages of Drosophila anteroposterior patterning, this transition is not well understood. By carefully analysing the spatiotemporal dynamics of pair-rule gene expression, we demonstrate that frequency-doubling is precipitated by multiple coordinated changes to the network of regulatory interactions between the pair-rule genes. We identify the broadly expressed but temporally patterned transcription factor, Odd-paired (Opa/Zic), as the cause of these changes, and show that the patterning of the even-numbered parasegment boundaries relies on Opa-dependent regulatory interactions. Our findings indicate that the pair-rule gene regulatory network has a temporally modulated topology, permitting the pair-rule genes to play stage-specific patterning roles.
Relationships
Is supplemented by: https://doi.org/10.5061/dryad.cg35k
Sponsorship
This work was supported by a BBSRC PhD studentship to Erik Clark.
Funder references
BBSRC (1221578)
Identifiers
External DOI: https://doi.org/10.7554/eLife.18215
This record's URL: https://www.repository.cam.ac.uk/handle/1810/260377
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International
Recommended or similar items
The following licence files are associated with this item: