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dc.contributor.authorClark, Eriken
dc.contributor.authorAkam, Michaelen
dc.date.accessioned2016-09-26T11:26:41Z
dc.date.available2016-09-26T11:26:41Z
dc.date.issued2016-08-15en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/260377
dc.description.abstractThe Drosophila embryo transiently exhibits a double-segment periodicity, defined by the expression of seven 'pair-rule' genes, each in a pattern of seven stripes. At gastrulation, interactions between the pair-rule genes lead to frequency doubling and the patterning of 14 parasegment boundaries. In contrast to earlier stages of Drosophila anteroposterior patterning, this transition is not well understood. By carefully analysing the spatiotemporal dynamics of pair-rule gene expression, we demonstrate that frequency-doubling is precipitated by multiple coordinated changes to the network of regulatory interactions between the pair-rule genes. We identify the broadly expressed but temporally patterned transcription factor, Odd-paired (Opa/Zic), as the cause of these changes, and show that the patterning of the even-numbered parasegment boundaries relies on Opa-dependent regulatory interactions. Our findings indicate that the pair-rule gene regulatory network has a temporally modulated topology, permitting the pair-rule genes to play stage-specific patterning roles.
dc.description.sponsorshipThis work was supported by a BBSRC PhD studentship to Erik Clark.
dc.languageengen
dc.language.isoenen
dc.publishereLife Sciences Publications Ltd
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleOdd-paired controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory networken
dc.typeArticle
prism.numbere18215en
prism.publicationDate2016en
prism.publicationNameeLifeen
prism.volume5en
dc.identifier.doi10.17863/CAM.4608
dcterms.dateAccepted2016-08-14en
rioxxterms.versionofrecord10.7554/eLife.18215en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-08-15en
dc.contributor.orcidClark, Erik [0000-0002-5588-796X]
dc.contributor.orcidAkam, Michael [0000-0003-0063-2297]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (1221578)
cam.issuedOnline2016-08-15en
datacite.issupplementedby.doi10.5061/dryad.cg35ken


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International