Large-Conductance Transmembrane Porin Made from DNA Origami
Bhamidimarri, Satya Prathyusha
American Chemical Society
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Göpfrich, K., Li, C., Ricci, M., Bhamidimarri, S. P., Yoo, J., Gyenes, B., Ohmann, A., et al. (2016). Large-Conductance Transmembrane Porin Made from DNA Origami. ACS Nano, 10 8207-8214. https://doi.org/10.1021/acsnano.6b03759
DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins’ large ionic conductance.
DNA origami, ionic current recordings, lipid membrane, molecular dynamics, synthetic porin
K.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge, and the Oppenheimer Ph.D. studentship; U.F.K. from an ERC Consolidator Grant (Designerpores 647144); and M.R. from the Early Postdoc Mobility fellowship of the Swiss National Science Foundation. A.A., J.Y., and C.Y.L. acknowledge support form the National Science Foundation under grants DMR-1507985, PHY-1430124, and EEC-1227034 and the supercomputer time provided through XSEDE Allocation grant MCA05S028 and the Blue Waters petascale supercomputer system (UIUC). M.W. and S.P.B. acknowledge support from Marie Skłodowska Curie Actions within the Initial Training Networks Translocation Network, project no. 607694.
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
External DOI: https://doi.org/10.1021/acsnano.6b03759
This record's URL: https://www.repository.cam.ac.uk/handle/1810/260447
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