Large-Conductance Transmembrane Porin Made from DNA Origami
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Authors
Göpfrich, Kerstin
Li, Chen-Yu
Ricci, Maria
Bhamidimarri, Satya Prathyusha
Yoo, Jejoong
Gyenes, Bertalan
Winterhalter, Mathias
Aksimentiev, Aleksei
Publication Date
2016-08-09Journal Title
ACS Nano
ISSN
1936-0851
Publisher
American Chemical Society
Volume
10
Pages
8207-8214
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Göpfrich, K., Li, C., Ricci, M., Bhamidimarri, S. P., Yoo, J., Gyenes, B., Ohmann, A., et al. (2016). Large-Conductance Transmembrane Porin Made from DNA Origami. ACS Nano, 10 8207-8214. https://doi.org/10.1021/acsnano.6b03759
Abstract
DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins’ large ionic conductance.
Keywords
DNA origami, ionic current recordings, lipid membrane, molecular dynamics, synthetic porin
Sponsorship
K.G.
acknowledges funding from the Winton Programme for the
Physics of Sustainability, Gates Cambridge, and the Oppenheimer
Ph.D. studentship; U.F.K. from an ERC Consolidator
Grant (Designerpores 647144); and M.R. from the Early
Postdoc Mobility fellowship of the Swiss National Science
Foundation. A.A., J.Y., and C.Y.L. acknowledge support form
the National Science Foundation under grants DMR-1507985,
PHY-1430124, and EEC-1227034 and the supercomputer time
provided through XSEDE Allocation grant MCA05S028 and
the Blue Waters petascale supercomputer system (UIUC).
M.W. and S.P.B. acknowledge support from Marie Skłodowska
Curie Actions within the Initial Training Networks Translocation
Network, project no. 607694.
Funder references
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
Identifiers
External DOI: https://doi.org/10.1021/acsnano.6b03759
This record's URL: https://www.repository.cam.ac.uk/handle/1810/260447
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International
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