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Large-Conductance Transmembrane Porin Made from DNA Origami.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Göpfrich, Kerstin 
Li, Chen-Yu 
Ricci, Maria 
Bhamidimarri, Satya Prathyusha 
Yoo, Jejoong 

Abstract

DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.

Description

Keywords

DNA origami, ionic current recordings, lipid membrane, molecular dynamics, synthetic porin, DNA, Lipid Bilayers, Molecular Dynamics Simulation, Nanotechnology, Porins

Journal Title

ACS Nano

Conference Name

Journal ISSN

1936-0851
1936-086X

Volume Title

10

Publisher

American Chemical Society (ACS)
Sponsorship
European Research Council (647144)
K.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge, and the Oppenheimer Ph.D. studentship; U.F.K. from an ERC Consolidator Grant (Designerpores 647144); and M.R. from the Early Postdoc Mobility fellowship of the Swiss National Science Foundation. A.A., J.Y., and C.Y.L. acknowledge support form the National Science Foundation under grants DMR-1507985, PHY-1430124, and EEC-1227034 and the supercomputer time provided through XSEDE Allocation grant MCA05S028 and the Blue Waters petascale supercomputer system (UIUC). M.W. and S.P.B. acknowledge support from Marie Skłodowska Curie Actions within the Initial Training Networks Translocation Network, project no. 607694.