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dc.contributor.authorCapalbo, Luisaen
dc.contributor.authorMela, Ioannaen
dc.contributor.authorAbad, MAen
dc.contributor.authorJeyaprakash, AAen
dc.contributor.authorEdwardson, Michaelen
dc.contributor.authorD'Avino, Paoloen
dc.date.accessioned2016-12-12T09:38:05Z
dc.date.available2016-12-12T09:38:05Z
dc.date.issued2016-10-26en
dc.identifier.issn2046-2441
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/261515
dc.description.abstractThe chromosomal passenger complex (CPC)-composed of Aurora B kinase, Borealin, Survivin and INCENP-surveys the fidelity of genome segregation throughout cell division. The CPC has been proposed to prevent polyploidy by controlling the final separation (known as abscission) of the two daughter cells via regulation of the ESCRT-III CHMP4C component. The molecular details are, however, still unclear. Using atomic force microscopy, we show that CHMP4C binds to and remodels membranes $\textit{in vitro}$. Borealin prevents the association of CHMP4C with membranes, whereas Aurora B interferes with CHMP4C's membrane remodelling activity. Moreover, we show that CHMP4C phosphorylation is not required for its assembly into spiral filaments at the abscission site and that two distinctly localized pools of phosphorylated CHMP4C exist during cytokinesis. We also characterized the CHMP4C interactome in telophase cells and show that the centralspindlin complex associates preferentially with unphosphorylated CHMP4C in cytokinesis. Our findings indicate that gradual dephosphorylation of CHMP4C triggers a 'relay' mechanism between the CPC and centralspindlin that regulates the timely distribution and activation of CHMP4C for the execution of abscission.
dc.description.sponsorshipThis work was supported by Cancer Research UK grant no. C12296/A12541 to P.P.D., who is also the recipient of the Maplethorpe Fellowship from Murray Edwards College, Cambridge, UK. I.M. and J.M.E. were supported by Biotechnology and Biological Research Council grant no. BB/J018236/1. A.A.J. is supported by Wellcome Trust Career Development grant no. 095822 and European Commission Career Integration grant no. 334291.
dc.languageENGen
dc.language.isoenen
dc.publisherRoyal Society Publishing
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAurora Ben
dc.subjectcell divisionen
dc.subjectcentralspindlinen
dc.subjectcytokinesisen
dc.subjectmembrane remodellingen
dc.subjectmidbodyen
dc.titleCoordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesisen
dc.typeArticle
prism.issueIdentifier10en
prism.number160248en
prism.publicationDate2016en
prism.publicationNameOpen Biologyen
prism.volume6en
dc.identifier.doi10.17863/CAM.6710
dcterms.dateAccepted2016-10-04en
rioxxterms.versionofrecord10.1098/rsob.160248en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-10-26en
dc.contributor.orcidMela, Ioanna [0000-0002-2914-9971]
dc.contributor.orcidD'Avino, Paolo [0000-0002-4773-6950]
dc.identifier.eissn2046-2441
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/J018236/1)
cam.issuedOnline2016-10-26en
datacite.issupplementedby.doi10.6084/m9.figshare.c.3517575en
cam.orpheus.successThu Jan 30 12:53:47 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International