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Impact of Collagen/Heparin Multilayers for Regulating Bone Cellular Functions

Accepted version
Peer-reviewed

Type

Article

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Authors

Ferreira, AM 
Gentile, P 
Toumpaniari, S 
Ciardelli, G 
Birch, MA 

Abstract

Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(l-lactic acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multilayer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy, and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organization and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilizing the fibrous collagen layer, and potentially impacting hMSCs activity.

Description

Keywords

collagen, heparin, layer-by-layer, mesenchymal stem cells, surface modification

Journal Title

ACS Applied Materials & Interfaces

Conference Name

Journal ISSN

1944-8244
1944-8252

Volume Title

8

Publisher

American Chemical Society
Sponsorship
Ana M. Ferreira thanks the Lagrange CRT for financing her research