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Genetically targeted 3D visualisation of $\textit{Drosophila}$ neurons under Electron Microscopy and X-Ray Microscopy using miniSOG

Published version
Peer-reviewed

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Authors

Ng, J 
Browning, A 
Lechner, L 
Terada, M 
Howard, G 

Abstract

Large dimension, high-resolution imaging is important for neural circuit visualisation as neurons have both long- and short-range patterns: from axons and dendrites to the numerous synapses at terminal endings. Electron Microscopy (EM) is the favoured approach for synaptic resolution imaging but how such structures can be segmented from high-density images within large volume datasets remains challenging. Fluorescent probes are widely used to localise synapses, identify cell-types and in tracing studies. The equivalent EM approach would benefit visualising such labelled structures from within sub-cellular, cellular, tissue and neuroanatomical contexts. Here we developed genetically-encoded, electron-dense markers using miniSOG. We demonstrate their ability in 1) labelling cellular sub-compartments of genetically-targeted neurons, 2) generating contrast under different EM modalities, and 3) segmenting labelled structures from EM volumes using computer-assisted strategies. We also tested non-destructive X-ray imaging on whole Drosophila brains to evaluate contrast staining. This enabled us to target specific regions for EM volume acquisition.

Description

Keywords

Animals, Animals, Genetically Modified, Axons, Dendrites, Drosophila, Image Enhancement, Imaging, Three-Dimensional, Microscopy, Electron, Neurons, X-Rays

Journal Title

Scientific Reports

Conference Name

Journal ISSN

2045-2322
2045-2322

Volume Title

6

Publisher

Nature Publishing Group
Sponsorship
European Research Council (649111)
This work was supported by European Research Council Starting Investigator and Consolidator grants, the European Molecular Biology Organization (EMBO) Young Investigator Programme, and Medical Research Council (MRC) Grant MC-U105188491 (G.S.X.E.J).