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dc.contributor.authorDigby, Ricken
dc.contributor.authorBravo, Diegoen
dc.contributor.authorPaulsen, Oleen
dc.contributor.authorMagloire, Ven
dc.date.accessioned2017-02-03T10:20:53Z
dc.date.available2017-02-03T10:20:53Z
dc.date.issued2017-02-01en
dc.identifier.issn0028-3908
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/262280
dc.description.abstractThe medial entorhinal cortex (mEC) is a key structure which controls the communication between the hippocampus and the neocortex. During slow-wave sleep, it stands out from other cortical regions by exhibiting persistent activity that outlasts neocortical Up states, decoupling the entorhinal cortex-hippocampal interaction from the neocortex. Here, we compared the mechanisms involved in the maintenance of the Up state in the barrel cortex (BC) and mEC using whole cell recordings in acute mouse brain slices. Bath application of an NMDA receptor antagonist abolished Up states in the BC, and reduced the incidence but not the duration of Up states in the mEC. Conversely, blockade of kainate receptors decreased Up state duration in the mEC, but not in the BC. Voltage clamp recordings demonstrated the presence of a non-NMDA glutamate receptor-mediated slow excitatory postsynaptic current, sensitive to the selective kainate receptor antagonist UBP-302, in layer III neurons of the mEC, which was not observed in the BC. Moreover, we found that kainate receptor-mediated currents assist in recovery back to the Up state membrane potential following a current-induced hyperpolarisation of individual cells in the mEC. Finally, we were able to generate Up state activity in a network model of exponential integrate-and-fire neurons only supported by AMPA and kainate receptor-mediated currents. We propose that synaptic kainate receptors are responsible for the unique properties of mEC Up states.
dc.description.sponsorshipWe also would like to acknowledge support from the Medical Research Council, UK. R.J.D. is on the Cambridge MB/PhD programme. D.S.B. is supported by the Gates Cambridge Trust. V.M. was supported by a Swiss National Science Foundation Early Postdoc Mobility Fellowship.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectUp stateen
dc.subjectSlow oscillationen
dc.subjectMedial entorhinal cortexen
dc.subjectBarrel cortexen
dc.subjectKainate receptoren
dc.subjectNMDA receptoren
dc.titleDistinct mechanisms of Up state maintenance in the medial entorhinal cortex and neocortexen
dc.typeArticle
prism.endingPage555
prism.publicationDate2017en
prism.publicationNameNeuropharmacologyen
prism.startingPage543
prism.volume113 Part Aen
dc.identifier.doi10.17863/CAM.7532
dcterms.dateAccepted2016-11-08en
rioxxterms.versionofrecord10.1016/j.neuropharm.2016.11.009en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-02-01en
dc.contributor.orcidPaulsen, Ole [0000-0002-2258-5455]
dc.identifier.eissn1873-7064
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2016-11-10en
cam.orpheus.successThu Jan 30 12:57:04 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International