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dc.contributor.authorScanlan, Een
dc.contributor.authorYu, Len
dc.contributor.authorMaskell, Duncanen
dc.contributor.authorChoudhary, Jen
dc.contributor.authorGrant, Andrewen
dc.date.accessioned2017-02-08T16:52:36Z
dc.date.available2017-02-08T16:52:36Z
dc.date.issued2017-01-30en
dc.identifier.issn1874-3919
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/262384
dc.description.abstract$\textit{Campylobacter jejuni}$ is the leading cause of bacterial gastroenteritis in the world. A number of factors are believed to contribute to the ability of $\textit{C. jejuni}$ to cause disease within the human host including the secretion of non-flagellar proteins via the flagellar type III secretion system (FT3SS). Here for the first time we have utilised quantitative proteomics using stable isotope labelling by amino acids in cell culture (SILAC), and label-free liquid chromatography-mass spectrometry (LC/MS), to compare supernatant samples from $\textit{C. jejuni}$ M1 wild type and flagella-deficient ($\textit{flgG}$mutant) strains to identify putative novel proteins secreted via the FT3SS. Genes encoding proteins that were candidates for flagellar secretion, derived from the LC/MS and SILAC datasets, were deleted. Infection of human CACO-2 tissue culture cells using these mutants resulted in the identification of novel genes required for interactions with these cells. This work has shown for the first time that both CJM1_0791 and CJM1_0395 are dependent on the flagellum for their presence in supernatants from $\textit{C. jejuni}$ stains M1 and 81-176. $\textit{BIOLOGICAL SIGNIFICANCE}$: This study provides the most complete description of the $\textit{Campylobacter jejuni}$ secretome to date. SILAC and label-free proteomics comparing mutants with or without flagella have resulted in the identification of two $\textit{C. jejuni}$ proteins that are dependent on flagella for their export from the bacterial cell.
dc.description.sponsorshipThis work was supported by an Isaac Newton Trust (reference 12.21(A)I)/Wellcome Trust ISSF (reference 097814/z/11/z)/University of Cambridge joint research grant awarded to AJG. JC and LY were supported by the Wellcome Trust grant number WT098051.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject$\textit{Campylobacter jejuni}$en
dc.subjectsecretomeen
dc.subjecttype III secretion systemen
dc.subjectFlagellaen
dc.subjectSILACen
dc.subjectmass specen
dc.titleA quantitative proteomic screen of the $\textit{Campylobacter jejuni}$ flagellar-dependent secretomeen
dc.typeArticle
prism.endingPage187
prism.publicationDate2017en
prism.publicationNameJournal of Proteomicsen
prism.startingPage181
prism.volume152en
dc.identifier.doi10.17863/CAM.7648
dcterms.dateAccepted2016-11-14en
rioxxterms.versionofrecord10.1016/j.jprot.2016.11.009en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-01-30en
dc.contributor.orcidMaskell, Duncan [0000-0002-5065-653X]
dc.contributor.orcidGrant, Andrew [0000-0001-9746-2989]
dc.identifier.eissn1876-7737
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2016-11-16en
cam.orpheus.successThu Jan 30 12:57:07 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International