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dc.contributor.authorMartins, Ten
dc.contributor.authorMeghini, Fen
dc.contributor.authorFlorio, Fen
dc.contributor.authorKimata, Yuuen
dc.date.accessioned2017-02-10T10:37:10Z
dc.date.available2017-02-10T10:37:10Z
dc.date.issued2017-01-09en
dc.identifier.issn1534-5807
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/262437
dc.description.abstractThe cell cycle is coordinated with differentiation during animal development. Here we report a cell-cycle-independent developmental role for a master cell-cycle regulator, the anaphase-promoting complex or cyclosome (APC/C), in the regulation of cell fate through modulation of Wingless (Wg) signaling. The APC/C controls both cell-cycle progression and postmitotic processes through ubiquitin-dependent proteolysis. Through an RNAi screen in the developing $\textit{Drosophila}$eye, we found that partial APC/C inactivation severely inhibits retinal differentiation independently of cell-cycle defects. The differentiation inhibition coincides with hyperactivation of Wg signaling caused by the accumulation of a Wg modulator, $\textit{Drosophila}$ Nek2 (dNek2). The APC/C degrades dNek2 upon synchronous G1 arrest prior to differentiation, which allows retinal differentiation through local suppression of Wg signaling. We also provide evidence that decapentaplegic signaling may posttranslationally regulate this APC/C function. Thus, the APC/C coordinates cell-fate determination with the cell cycle through the modulation of developmental signaling pathways.
dc.description.sponsorshipT.M. and F.M. were partly supported by a CRUK Fellowship to Y.K. T.M. thanks the European Commission for a Marie Curie fellowship.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAPC/Cen
dc.subjectproteolysisen
dc.subjectwinglessen
dc.subjectWnten
dc.subjectDrosophilaen
dc.subjectNek2en
dc.subjecteye developmenten
dc.subjectDppen
dc.subjectdifferentiationen
dc.subjectfate specificationen
dc.titleThe APC/C Coordinates Retinal Differentiation with G1 Arrest through the Nek2-Dependent Modulation of Wingless Signalingen
dc.typeArticle
prism.endingPage80
prism.issueIdentifier1en
prism.publicationDate2017en
prism.publicationNameDevelopmental Cellen
prism.startingPage67
prism.volume40en
dc.identifier.doi10.17863/CAM.7701
dcterms.dateAccepted2016-11-15en
rioxxterms.versionofrecord10.1016/j.devcel.2016.12.005en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-01-09en
dc.contributor.orcidKimata, Yuu [0000-0001-6223-1320]
dc.identifier.eissn1878-1551
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (12874)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (655297)
cam.issuedOnline2016-12-29en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International