Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
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Peer-reviewed
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Article
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Abstract
Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.
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Keywords
Annexin A5, Apoptosis, Boronic Acids, Fluorescent Dyes, HeLa Cells, Humans, Hydrazones, Microscopy, Confocal, Models, Molecular, Molecular Conformation
Journal Title
Chemical Communications
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Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
53
Publisher
Royal Society of Chemistry
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Sponsorship
Engineering and Physical Sciences Research Council (EP/M003647/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (675007)
European Research Council (676832)
European Commission (EC) (852985)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (675007)
European Research Council (676832)
European Commission (EC) (852985)
We thank FCT Portugal (Doctoral Fellowship, SFRH/BD/94779/2013 to F. M. F. S., Postdoctoral Fellowship, SFRH/BPD/103172/2014 to P. M. S. D. C.; projects PTDC/QUI-QUI/118315/2010 and PTDC/BBB BQB/0506/2012; PTDC/QEQ-QOR/1434/2014: PTDC/SAUFAR/119389/2010; FCT Investigator to G. J. L. B. and P. M. P. G.; iMed.ULisboa grant UID/DTP/04138/2013), EU (Marie-Curie CIG to G. J. L. B.; Marie-Sklodowska Curie ITN ProteinConjugates to G. J. L. B. and P. M. P. G.), DFG (SI 2117/1-1 to F. S.), CNPq Brazil (fellowship 200456/2015-6 to J. B. B.); Ministerio de Economía y Competitividad, Madrid, Spain (grant CTQ2014-54729-C2-1-P), Junta de Andalucía (grant P12-FQM-2140) and the EPSRC (G. J. L. B.) for financial support. G. J. L. B. is a Royal Society University Research Fellow and the recipient of a European Research Council Starting Grant (TagIt).