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High Performance Reduction of H2O2 with an Electron Transport Decaheme Cytochrome on a Porous ITO Electrode

Accepted version
Peer-reviewed

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Authors

Reuillard, Bertrand  ORCID logo  https://orcid.org/0000-0002-9553-1773
Ly, K 
Hildebrandt, P 
Jeuken, LJC 

Abstract

The decaheme cytochrome MtrC from Shewanella oneidensis MR-1 immobilized on an ITO electrode displays unprecedented H2O2 reduction activity. Although MtrC showed lower peroxidase activity in solution compared to horseradish peroxidase, the ten heme cofactors enable excellent electronic communication and a superior activity on the electrode surface. A hierarchical ITO electrode enabled optimal immobilization of MtrC and a high current density of 1 mA cm−2 at 0.4 Vvs SHE could be obtained at pH 6.5 (Eonset = 0.72 V). UV−visible and Resonance Raman spectroelectrochemical studies suggest the formation of a high valent iron-oxo species as the catalytic intermediate. Our findings demonstrate the potential of multiheme cytochromes to catalyze technologically relevant reactions and establish MtrC as a new benchmark in biotechnological H2O2 reduction with scope for applications in fuel cells and biosensors.

Description

Keywords

34 Chemical Sciences

Journal Title

Journal of the American Chemical Society

Conference Name

Journal ISSN

0002-7863
1520-5126

Volume Title

139

Publisher

American Chemical Society
Sponsorship
European Research Council (682833)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (701192)
Biotechnology and Biological Sciences Research Council (BB/K010220/1)
Biotechnology and Biological Sciences Research Council (BB/J000124/1)
BBSRC, ERC, DFG, Marie Sklodowska Curie fellowship
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