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dc.contributor.authorAndersson Rolf, Amandaen
dc.contributor.authorMustata, RCen
dc.contributor.authorMerenda, Allessandraen
dc.contributor.authorKim, Jihoonen
dc.contributor.authorPerera, Sen
dc.contributor.authorGrego, Ten
dc.contributor.authorAndrews, Ken
dc.contributor.authorTremble, Ken
dc.contributor.authorRebelo Da Silva, Joseen
dc.contributor.authorFink, Juergenen
dc.contributor.authorSkarnes, WCen
dc.contributor.authorKoo, Bonkyoungen
dc.date.accessioned2017-03-13T15:02:16Z
dc.date.available2017-03-13T15:02:16Z
dc.date.issued2017-03-01en
dc.identifier.issn1548-7091
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/263063
dc.description.abstractLoss-of-function studies are key for investigating gene function, and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem cells, 3D organoids and cell lines, by co-delivery of CRISPR-Cas9 and a universal conditional intronic cassette.
dc.description.sponsorshipA.A.-R. and K.T. are supported by the Medical Research Council, A.M. is supported by Wntsapp, Marie Curie ITN. J.F. and J.C.R.S. are supported by the Wellcome Trust. W.C.S. received core grant support from the Wellcome Trust to the Wellcome Trust Sanger Institute. B.-K.K. and R.C.M. are supported by a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society (101241/Z/13/Z) and receive a core support grant from the Wellcome Trust and MRC to the WT–MRC Cambridge Stem Cell Institute.
dc.languageengen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.titleOne-step generation of conditional and reversible gene knockoutsen
dc.typeArticle
prism.publicationDate2017en
prism.publicationNameNature Methodsen
dc.identifier.doi10.17863/CAM.8363
dcterms.dateAccepted2016-12-21en
rioxxterms.versionofrecord10.1038/nmeth.4156en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-03-01en
dc.contributor.orcidRebelo Da Silva, Jose [0000-0001-5487-1117]
dc.identifier.eissn1548-7105
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_PC_12009)
pubs.funder-project-idWellcome Trust (101861/Z/13/Z)
pubs.funder-project-idWellcome Trust (097922/B/11/Z)
pubs.funder-project-idWellcome Trust (101241/Z/13/Z)
cam.issuedOnline2017-01-30en
rioxxterms.freetoread.startdate2017-07-30


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