Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.
Royal Society of Chemistry
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Kawasaki, F., Murat, P., Li, Z., Santner, T., & Balasubramanian, S. (2017). Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.. Chemical Communications, 53 1389-1392. https://doi.org/10.1039/c6cc08670e
We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.
This work was supported by the following grants: the Wellcome Trust (099232/Z/12/Z), Cancer Research UK (C14303/A17197), ERC Advanced grant (PM), Herchel Smith Funding and A*STAR (ZL).
Wellcome Trust (099232/Z/12/Z)
External DOI: https://doi.org/10.1039/c6cc08670e
This record's URL: https://www.repository.cam.ac.uk/handle/1810/263112