Taking the strain: quantifying the contributions of all cell behaviours to changes in epithelial shape
Philosophical Transactions of the Royal Society B: Biological Sciences
Royal Society Publishing
MetadataShow full item record
Blanchard, G. (2017). Taking the strain: quantifying the contributions of all cell behaviours to changes in epithelial shape. Philosophical Transactions of the Royal Society B: Biological Sciences, 372 (1720. 20150513)https://doi.org/10.1098/rstb.2015.0513
Computer-assisted tracking of the shapes of many cells over long periods of development has driven the exploration of novel ways to quantify the contributions of different cell behaviours to morphogenesis. A handful of similar methods have now been published that are used to calculate tissue deformations (strain rates) in epithelia. These methods are further used to quantify strain rates attributable to each of the cell behaviours in the tissue, such as cell shape change, cell rearrangement and cell division, that together sum to the tissue strain rates. In this review, aimed at developmental biologists, I will introduce the general approach, characterize differences in current approaches and highlight extensions of these methods that remain to be fully explored. The methods will make a major contribution to the emerging field of tissue mechanics. Precisely quantified strain rates are an essential first step towards exploring constitutive equations relating stress to strain via tissue mechanical properties. This article is part of the themed issue ‘Systems morphodynamics: understanding the development of tissue hardware’.
epithelial morphogenesis, cell tracking, kinematic strain rates, simple shear, tissue mechanics, mechanical inference
G.B.B. was supported by grant no. 15.23(k) from the Isaac Newton Trust and by Wellcome Trust grant no. 100329/Z/12/Z awarded to Prof. William Harris.
Wellcome Trust (099234/Z/12/Z)
Wellcome Trust (100329/Z/12/Z)
External DOI: https://doi.org/10.1098/rstb.2015.0513
This record's URL: https://www.repository.cam.ac.uk/handle/1810/263261