Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity
Authors
Thomas, David
Clare, Simon
Sowerby, John
Pardo, M
Juss, JK
Goulding, DA
van der Weyden, L
Storisteanu, D
Prakash, A
Espéli, M
Flint, S
Hoenderdos, K
Kane, L
Harcourt, K
Mukhopadhyay, S
Umrania, Yagnesh
Antrobus, R
Adams, DJ
Bateman, A
Choudhary, JS
Condliffe, AM
Publication Date
2017-04-03Journal Title
The Journal of Experimental Medicine
ISSN
0022-1007
Publisher
Rockefeller University Press
Volume
214
Issue
4
Pages
1111-1128
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Thomas, D., Clare, S., Sowerby, J., Pardo, M., Juss, J., Goulding, D., van der Weyden, L., et al. (2017). Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. The Journal of Experimental Medicine, 214 (4), 1111-1128. https://doi.org/10.1084/jem.20161382
Abstract
The phagocyte respiratory burst is crucial for innate immunity. The transfer of electrons to oxygen is mediated by a membrane-bound heterodimer, comprising gp91$\textit{phox}$ and p22$\textit{phox}$ subunits. Deficiency of either subunit leads to severe immunodeficiency. We describe Eros (essential for reactive oxygen species), a protein encoded by the previously undefined mouse gene $\textit{bc017643}$, and show that it is essential for host defense via the phagocyte NAPDH oxidase. Eros is required for expression of the NADPH oxidase components, gp91$\textit{phox}$ and p22$\textit{phox}$. Consequently, $\textit{Eros}$-deficient mice quickly succumb to infection. $\textit{Eros}$ also contributes to the formation of neutrophil extracellular traps (NETS) and impacts on the immune response to melanoma metastases. $\textit{Eros}$ is an ortholog of the plant protein Ycf4, which is necessary for expression of proteins of the photosynthetic photosystem 1 complex, itself also an NADPH oxio-reductase. We thus describe the key role of the previously uncharacterized protein Eros in host defense.
Sponsorship
D.C. Thomas was funded by a Wellcome Trust/CIMR Next Generation Fellowship, a National Institute for Health Research (NIHR) Clinical Lectureship, and a Starter Grant for Clinical Lecturers (Academy of Medical Sciences). K.G.C. Smith was funded by funded by the Medical Research Council (program grant MR/L019027) and is a Wellcome Investigator and a NIHR Senior Investigator. S. Clare and G. Dougan were funded by the Wellcome Trust (grant 098051). The Cambridge Institute for Medical Research is in receipt of a Wellcome Trust Strategic Award (079895). J.C.L is funded by a Wellcome Intermediate Clinical Fellowship 105920/2/14/2.
Funder references
Wellcome Trust (102770/Z/13/Z)
MRC (MR/L019027/1)
WELLCOME TRUST (105920/Z/14/Z)
Wellcome Trust (079895/Z/06/B)
Identifiers
External DOI: https://doi.org/10.1084/jem.20161382
This record's URL: https://www.repository.cam.ac.uk/handle/1810/263481
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International