Show simple item record

dc.contributor.authorGuinane, CMen
dc.contributor.authorBen Zakour, NLen
dc.contributor.authorTormo-Mas, MAen
dc.contributor.authorWeinert, Lucyen
dc.contributor.authorLowder, BVen
dc.contributor.authorCartwright, RAen
dc.contributor.authorSmyth, DSen
dc.contributor.authorSmyth, CJen
dc.contributor.authorLindsay, JAen
dc.contributor.authorGould, KAen
dc.contributor.authorWitney, Aen
dc.contributor.authorHinds, Jen
dc.contributor.authorBollback, JPen
dc.contributor.authorRambaut, Aen
dc.contributor.authorPenadés, JRen
dc.contributor.authorFitzgerald, JRen
dc.date.accessioned2017-04-12T13:21:06Z
dc.date.available2017-04-12T13:21:06Z
dc.date.issued2010-07-12en
dc.identifier.issn1759-6653
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/263635
dc.description.abstractPhenotypic biotyping has traditionally been used to differentiate bacteria occupying distinct ecological niches such as host species. For example, the capacity of Staphylococcus aureus from sheep to coagulate ruminant plasma, reported over 60 years ago, led to the description of small ruminant and bovine S. aureus ecovars. The great majority of small ruminant isolates are represented by a single, widespread clonal complex (CC133) of S. aureus, but its evolutionary origin and the molecular basis for its host tropism remain unknown. Here, we provide evidence that the CC133 clone evolved as the result of a human to ruminant host jump followed by adaptive genome diversification. Comparative whole-genome sequencing revealed molecular evidence for host adaptation including gene decay and diversification of proteins involved in host-pathogen interactions. Importantly, several novel mobile genetic elements encoding virulence proteins with attenuated or enhanced activity in ruminants were widely distributed in CC133 isolates, suggesting a key role in its host-specific interactions. To investigate this further, we examined the activity of a novel staphylococcal pathogenicity island (SaPIov2) found in the great majority of CC133 isolates which encodes a variant of the chromosomally encoded von Willebrand-binding protein (vWbp(Sov2)), previously demonstrated to have coagulase activity for human plasma. Remarkably, we discovered that SaPIov2 confers the ability to coagulate ruminant plasma suggesting an important role in ruminant disease pathogenesis and revealing the origin of a defining phenotype of the classical S. aureus biotyping scheme. Taken together, these data provide broad new insights into the origin and molecular basis of S. aureus ruminant host specificity.
dc.description.sponsorshipThis work was funded by grant BB/D521222/1 from the Biotechnology and Biological Sciences Research Council (to J.R.F.). The Bacterial Microarray Group at St Georges is funded by The Wellcome Trust.
dc.languageengen
dc.language.isoenen
dc.publisherOxford University Press
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectAdaptation, Physiologicalen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectCattleen
dc.subjectComparative Genomic Hybridizationen
dc.subjectDNA, Bacterialen
dc.subjectEcosystemen
dc.subjectEvolution, Molecularen
dc.subjectGenome, Bacterialen
dc.subjectGoatsen
dc.subjectHost-Pathogen Interactionsen
dc.subjectHumansen
dc.subjectInterspersed Repetitive Sequencesen
dc.subjectPhylogenyen
dc.subjectRuminantsen
dc.subjectSheepen
dc.subjectSpecies Specificityen
dc.subjectStaphylococcus aureusen
dc.titleEvolutionary genomics of Staphylococcus aureus reveals insights into the origin and molecular basis of ruminant host adaptationen
dc.typeArticle
prism.endingPage466
prism.publicationDate2010en
prism.publicationNameGenome Biology and Evolutionen
prism.startingPage454
prism.volume2en
dc.identifier.doi10.17863/CAM.8994
dcterms.dateAccepted2010-06-04en
rioxxterms.versionofrecord10.1093/gbe/evq031en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc/4.0/en
rioxxterms.licenseref.startdate2010-07-12en
dc.contributor.orcidWeinert, Lucy [0000-0002-9279-6012]
dc.identifier.eissn1759-6653
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2010-06-09en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 4.0 International