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dc.contributor.authorStroehl, Florianen
dc.contributor.authorLin, Qiaojinen
dc.contributor.authorLaine, RFen
dc.contributor.authorWong, HH-Wen
dc.contributor.authorUrbančič, Ven
dc.contributor.authorCagnetta, Robertaen
dc.contributor.authorHolt, Christineen
dc.contributor.authorKaminski, Clemensen
dc.date.accessioned2017-04-12T15:21:12Z
dc.date.available2017-04-12T15:21:12Z
dc.date.issued2017-04-06en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/263650
dc.description.abstractLocal mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.
dc.description.sponsorshipThis work was supported by grants from the Leverhulme Trust, the Engineering and Physical Sciences Research Council, UK (grant EP/H018301/1), the Medical Research Council (grant MR/K015850/1, and MR/K02292X/1), the Wellcome Trust (089703/Z/09/Z) (C.F.K.), Sir Edward Youde Memorial Fund, Croucher Foundation, Cambridge Trust (H.H.W.) Gates Cambridge Scholarship (J.Q.L.), Wellcome Trust Studentship (V.U.), European Research Council Advanced Grant (322817), the Wellcome Trust (085314/Z/08/Z) (C.E.H.).
dc.languageengen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectaxon and dendritic guidanceen
dc.subjectmicroscopyen
dc.titleSingle Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axonsen
dc.typeArticle
prism.issueIdentifier1en
prism.number709en
prism.publicationDate2017en
prism.publicationNameScientific Reportsen
prism.volume7en
dc.identifier.doi10.17863/CAM.9008
dcterms.dateAccepted2017-03-08en
rioxxterms.versionofrecord10.1038/s41598-017-00695-7en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-04-06en
dc.contributor.orcidStroehl, Florian [0000-0002-2603-0780]
dc.contributor.orcidLin, Qiaojin [0000-0002-2669-6478]
dc.contributor.orcidHolt, Christine [0000-0003-2829-121X]
dc.contributor.orcidKaminski, Clemens [0000-0002-5194-0962]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (322817)
pubs.funder-project-idMRC (MR/K02292X/1)
pubs.funder-project-idMRC (MR/K015850/1)
pubs.funder-project-idWellcome Trust (085314/Z/08/Z)
pubs.funder-project-idWellcome Trust (089703/Z/09/Z)
pubs.funder-project-idBBSRC (BB/H023917/1)
pubs.funder-project-idEPSRC (EP/H018301/1)
pubs.funder-project-idMRC (G0902243)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International