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dc.contributor.authorJaneczek, AAen
dc.contributor.authorScarpa, Een
dc.contributor.authorHorrocks, Mathewen
dc.contributor.authorTare, RSen
dc.contributor.authorRowland, CAen
dc.contributor.authorJenner, Den
dc.contributor.authorNewman, TAen
dc.contributor.authorOreffo, ROen
dc.contributor.authorLee, Stevenen
dc.contributor.authorEvans, NDen
dc.date.accessioned2017-04-28T15:12:31Z
dc.date.available2017-04-28T15:12:31Z
dc.date.issued2017-04-01en
dc.identifier.issn1743-5889
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/263891
dc.description.abstractAim: To fabricate PEGylated liposomes which preserve the activity of hydrophobic Wnt3A protein, and to demonstrate their efficacy in promoting expansion of osteoprogenitors from human bone marrow. Methods: PEGylated liposomes composed of several synthetic lipids were tested for their ability to preserve Wnt3A activity in reporter and differentiation assays. Single-molecule microspectroscopy was used to test for direct association of protein with liposomes. Results: Labeled Wnt3A protein directly associated with all tested liposome preparations. However, Wnt3A activity was preserved or enhanced in PEGylated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes but not in PEGylated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes. PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis. Conclusion: Active Wnt protein-containing PEGylated liposomes may have utility for systemic administration for bone repair.
dc.description.sponsorshipThe authors acknowledge funding support from the Medical Research Council, UK (grant number MR/J004103/1), Wessex Medical Research (grant number SO2), UoS Research Management Committee and the Institute for Life Sciences, Southampton. The authors would like to thank the Royal Society for the University Research Fellowship of Steven F Lee (UF120277).
dc.languageengen
dc.language.isoenen
dc.publisherFuture Medicine Ltd.
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPEGylated liposomesen
dc.subjectWnten
dc.subjectfracture repairen
dc.subjectliposomesen
dc.subjectmarrow stromal cellsen
dc.subjectmesenchymal stem cellsen
dc.subjectosteoprogenitorsen
dc.subjectregenerative medicineen
dc.subjectskeletal stem cellsen
dc.titlePEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populationsen
dc.typeArticle
prism.endingPage863
prism.issueIdentifier8en
prism.publicationDate2017en
prism.publicationNameNanomedicineen
prism.startingPage845
prism.volume12en
dc.identifier.doi10.17863/CAM.9269
dcterms.dateAccepted2017-02-07en
rioxxterms.versionofrecord10.2217/nnm-2016-0386en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-04-01en
dc.contributor.orcidLee, Steven [0000-0003-4492-5139]
dc.identifier.eissn1748-6963
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idRoyal Society (uf120277)
cam.issuedOnline2017-03-29en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International