Lung Organoids and Their Use To Study Cell-Cell Interaction
Journal Title
Current Pathobiology Reports
ISSN
2167-485X
Publisher
Springer
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Nikolić, M., & Rawlins, E. (2017). Lung Organoids and Their Use To Study Cell-Cell Interaction. Current Pathobiology Reports https://doi.org/10.1007/s40139-017-0137-7
Abstract
$\textit{Purpose of Review}$ The lung research field has pioneered the use of organoids for the study of cell-cell interactions.
$\textit{Recent Findings}$ The use of organoids for airway basal cells is routine. However, the development of organoids for the other regions of the lung is still in its infancy. Such cultures usually rely on cell-cell interactions between the stem cells and a putative niche cell for their growth and differentiation.
$\textit{Summary}$ The use of co-culture organoid systems has facilitated the in vitro cultivation of previously inaccessible stem cell populations, providing a novel method for dissecting the molecular requirements of these cell-cell interactions. Future technology development will allow the growth of epithelial-only organoids in more defined media and also the introduction of specific non-epithelial cells for the study of cell interactions. These developments will require an improved understanding of the epithelial and non-epithelial cell types present in the lung and their lineage relationships.
Keywords
organoids, lung progenitors, mouse lung, human lung, iPSCs
Sponsorship
Wellcome Trust PhD Programme for Clinicians to MZN, Medical Research Council G0900424 to ELR.
Funder references
WELLCOME TRUST (105602/Z/14/Z)
MRC (G0900424)
Wellcome Trust (092096/Z/10/Z)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1007/s40139-017-0137-7
This record's URL: https://www.repository.cam.ac.uk/handle/1810/264128
Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International