Repository logo
 

Lung Organoids and Their Use To Study Cell-Cell Interaction

Published version
Peer-reviewed

Change log

Authors

Nikolić, MZ 
Rawlins, EL 

Abstract

Purpose of Review The lung research field has pioneered the use of organoids for the study of cell-cell interactions.

Recent Findings The use of organoids for airway basal cells is routine. However, the development of organoids for the other regions of the lung is still in its infancy. Such cultures usually rely on cell-cell interactions between the stem cells and a putative niche cell for their growth and differentiation.

Summary The use of co-culture organoid systems has facilitated the in vitro cultivation of previously inaccessible stem cell populations, providing a novel method for dissecting the molecular requirements of these cell-cell interactions. Future technology development will allow the growth of epithelial-only organoids in more defined media and also the introduction of specific non-epithelial cells for the study of cell interactions. These developments will require an improved understanding of the epithelial and non-epithelial cell types present in the lung and their lineage relationships.

Description

Keywords

organoids, lung progenitors, mouse lung, human lung, iPSCs

Journal Title

Current Pathobiology Reports

Conference Name

Journal ISSN

2167-485X
2167-485X

Volume Title

Publisher

Springer
Sponsorship
Wellcome Trust (105602/Z/14/Z)
Medical Research Council (G0900424)
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust PhD Programme for Clinicians to MZN, Medical Research Council G0900424 to ELR.