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dc.contributor.authorLam, Brianen
dc.contributor.authorCimino, Ireneen
dc.contributor.authorPolex-Wolf, Jen
dc.contributor.authorNicole Kohnke, Sen
dc.contributor.authorRimmington, Den
dc.contributor.authorIyemere, Ven
dc.contributor.authorHeeley, Nen
dc.contributor.authorCossetti, Cen
dc.contributor.authorSchulte, Ren
dc.contributor.authorSaraiva, LRen
dc.contributor.authorLogan, DWen
dc.contributor.authorBlouet, Clemenceen
dc.contributor.authorO'Rahilly, Stephenen
dc.contributor.authorColl, Anthonyen
dc.contributor.authorYeo, Gilesen
dc.date.accessioned2017-05-08T11:02:56Z
dc.date.available2017-05-08T11:02:56Z
dc.date.issued2017-05-01en
dc.identifier.issn2212-8778
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264134
dc.description.abstract$\textbf{Objective}$ Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture. $\textbf{Methods}$ Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons. $\textbf{Results}$ Of 163 neurons, 118 expressed high levels of $\textit{Pomc}$ with little/no Agrp expression and were considered “canonical” POMC neurons (P$^{+}$). The other 45/163 expressed low levels of $\textit{Pomc}$ and high levels of $\textit{Agrp}$ (A$^{+}$P$_{+}$). Unbiased clustering analysis of P$^{+}$ neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P$^{+}$ neurons expressed the leptin receptor ($\textit{Lepr}$) compared with 58% (26/45) of A$^{+}$P$_{+}$ neurons. In contrast, the insulin receptor ($\textit{Insr}$) was expressed at similar frequency on P$^{+}$ and A$^{+}$P$_{+}$ neurons (64% and 55%, respectively). $\textbf{Conclusion}$ These data reveal arcuate POMC neurons to be a highly heterogeneous population. Accession Numbers: GSE92707.
dc.description.sponsorshipThis work was supported by the UK Medical Research Council (MRC) Metabolic Disease Unit (MRC_MC_UU_12012/1 & MRC_MC_UU_12012/5), a Wellcome Trust Strategic Award (100574/Z/12/Z), and the Helmholtz Alliance ICEMED.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPOMCen
dc.subjectmelanocortinen
dc.subjectAGRPen
dc.subjectleptinen
dc.subjectinsulinen
dc.subjecthypothalamusen
dc.subjectarcuate nucleusen
dc.subjectgene expressionen
dc.subjectneuronen
dc.subjecttranscriptomeen
dc.titleHeterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencingen
dc.typeArticle
prism.endingPage392
prism.issueIdentifier5en
prism.publicationDate2017en
prism.publicationNameMolecular Metabolismen
prism.startingPage383
prism.volume6en
dc.identifier.doi10.17863/CAM.9497
dcterms.dateAccepted2017-02-23en
rioxxterms.versionofrecord10.1016/j.molmet.2017.02.007en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-05-01en
dc.contributor.orcidLam, Brian [0000-0002-3638-9025]
dc.contributor.orcidCimino, Irene [0000-0003-1397-5408]
dc.contributor.orcidBlouet, Clemence [0000-0002-1752-1270]
dc.contributor.orcidO'Rahilly, Stephen [0000-0003-2199-4449]
dc.contributor.orcidColl, Anthony [0000-0003-2594-7463]
dc.contributor.orcidYeo, Giles [0000-0001-8823-3615]
dc.identifier.eissn2212-8778
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_UU_12012/1)
pubs.funder-project-idMRC (MC_UU_12012/5)
pubs.funder-project-idBBSRC (1644127)
pubs.funder-project-idWellcome Trust (100574/Z/12/Z)
pubs.funder-project-idMEDICAL RESEARCH COUNCIL (G0900554)
cam.issuedOnline2017-03-01en
cam.orpheus.successThu Jan 30 12:54:03 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International