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dc.contributor.authorCharbonneau, Amelia RLen
dc.contributor.authorForman, Oliver Pen
dc.contributor.authorCain, Amy Ken
dc.contributor.authorNewland, Grahamen
dc.contributor.authorRobinson, Carlen
dc.contributor.authorBoursnell, Mikeen
dc.contributor.authorParkhill, Julianen
dc.contributor.authorLeigh, James Aen
dc.contributor.authorMaskell, Duncanen
dc.contributor.authorWaller, Andrew Sen
dc.date.accessioned2017-06-01T06:02:24Z
dc.date.available2017-06-01T06:02:24Z
dc.date.issued2017-05-31en
dc.identifier.citationBMC Genomics. 2017 May 31;18(1):426
dc.identifier.issn1471-2164
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264558
dc.description.abstractAbstract Background Utilising next generation sequencing to interrogate saturated bacterial mutant libraries provides unprecedented information for the assignment of genome-wide gene essentiality. Exposure of saturated mutant libraries to specific conditions and subsequent sequencing can be exploited to uncover gene essentiality relevant to the condition. Here we present a barcoded transposon directed insertion-site sequencing (TraDIS) system to define an essential gene list for Streptococcus equi subsp. equi, the causative agent of strangles in horses, for the first time. The gene essentiality data for this group C Streptococcus was compared to that of group A and B streptococci. Results Six barcoded variants of pGh9:ISS1 were designed and used to generate mutant libraries containing between 33,000-66,000 unique mutants. TraDIS was performed on DNA extracted from each library and data were analysed separately and as a combined master pool. Gene essentiality determined that 19.5% of the S. equi genome was essential. Gene essentialities were compared to those of group A and group B streptococci, identifying concordances of 90.2% and 89.4%, respectively and an overall concordance of 83.7% between the three species. Conclusions The use of barcoded pGh9:ISS1 to generate mutant libraries provides a highly useful tool for the assignment of gene function in S. equi and other streptococci. The shared essential gene set of group A, B and C streptococci provides further evidence of the close genetic relationships between these important pathogenic bacteria. Therefore, the ABC of gene essentiality reported here provides a solid foundation towards reporting the functional genome of streptococci.
dc.titleDefining the ABC of gene essentiality in streptococcien
dc.typeArticle
dc.date.updated2017-06-01T06:02:24Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
prism.publicationDate2017en
dc.identifier.doi10.17863/CAM.10126
dcterms.dateAccepted2017-05-14en
rioxxterms.versionofrecord10.1186/s12864-017-3794-3en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-05-31en
dc.contributor.orcidParkhill, Julian [0000-0002-7069-5958]
dc.contributor.orcidMaskell, Duncan [0000-0002-5065-653X]
dc.identifier.eissn1471-2164
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMedical Research Council (G1100100)


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