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dc.contributor.authorRůžička, Ken
dc.contributor.authorZhang, Men
dc.contributor.authorCampilho, Aen
dc.contributor.authorBodi, Zen
dc.contributor.authorKashif, Men
dc.contributor.authorSaleh, Men
dc.contributor.authorEeckhout, Den
dc.contributor.authorEl-Showk, Sen
dc.contributor.authorLi, Hen
dc.contributor.authorZhong, Sen
dc.contributor.authorJaeger, GDen
dc.contributor.authorMongan, NPen
dc.contributor.authorHejátko, Jen
dc.contributor.authorHelariutta, Yrjoen
dc.contributor.authorFray, RGen
dc.date.accessioned2017-06-06T15:43:55Z
dc.date.available2017-06-06T15:43:55Z
dc.date.issued2017-07-01en
dc.identifier.issn0028-646X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264667
dc.description.abstract$\textit{N}$6-adenosine methylation m$^{6}$A of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m$^{6}$A writer proteins in $\textit{Arabidopsis thaliana}$. The components required for m$^{6}$A in $\textit{Arabidopsis}$ included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m$^{6}$A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m$^{6}$A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m$^{6}$A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
dc.description.sponsorshipSupported by the Czech Science Foundation (P501/12/0934), European Social Fund (CZ.1.07/2.3.00/20.0189), the Ministry of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability, NPUII-LQ1601 the NCLG Research Infrastructure, LM2015091, and the Czech-Biomaging, LM2015062) to K.R., M.S. and J.H. RNA methylation work in R.G.F.'s lab was supported by the Biotechnology and Biological Sciences Research Council (grant nos. BB/K013637/1 and BB/M008606/1). Y.H.'s laboratory was funded by the Gatsby Foundation (GAT3395/PR3); the National Science Foundation Biotechnology and Biological Sciences Research Council grant (BB/N013158/1); University of Helsinki (award 799992091), the European Research Council Advanced Investigator Grant SYMDEV (no. 323052); the Finnish Funding Agency for Technology and Innovation (Tekes); and the Academy of Finland Centre of Excellence program (award 63053034).
dc.languageengen
dc.language.isoenen
dc.publisherWiley
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectArabidopsisen
dc.subjectHAKAIen
dc.subjectN6-adenosine methylation (m6A)en
dc.subjectVIRILIZERen
dc.subjectmRNA methylationen
dc.subjectprotoxylemen
dc.titleIdentification of factors required for m$^{6}$A mRNA methylation in $\textit{Arabidopsis}$ reveals a role for the conserved E3 ubiquitin ligase HAKAI.en
dc.typeArticle
prism.endingPage172
prism.issueIdentifier1en
prism.publicationDate2017en
prism.publicationNameNew Phytologisten
prism.startingPage157
prism.volume215en
dc.identifier.doi10.17863/CAM.10263
dcterms.dateAccepted2017-03-20en
rioxxterms.versionofrecord10.1111/nph.14586en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-07-01en
dc.contributor.orcidHelariutta, Yrjo [0000-0002-7287-8459]
dc.identifier.eissn1469-8137
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/N013158/1)
pubs.funder-project-idEuropean Research Council (323052)
cam.issuedOnline2017-05-15en
cam.orpheus.successThu Jan 30 12:53:47 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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