Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition
eLife Sciences Publications Ltd
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Huang, Y., Kim, J., Do, D., Lee, C., Penfold, C., Zylicz, J., Marioni, J., et al. (2017). Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition. eLife, 6 (e22345)https://doi.org/10.7554/eLife.22345
The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.
2-cell, MuERV-L/MERVL, Stella, chimeric transcripts, developmental biology, maternal-to-zygotic transition, mouse, stem cells, transposable elements
The work was funded by a studentship to YH from the James Baird Fund, University of Cambridge, by the DGIST Start-up Fund of the Ministry of Science, ICT and Future Planning to JKK, by a core grant from EMBL and CRUK to JCM, by a Wellcome Trust Senior Investigator Award to MAS, and by a core grant from the Wellcome Trust and Cancer Research UK to the Gurdon Institute.
Wellcome Trust (096738/Z/11/Z)
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
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External DOI: https://doi.org/10.7554/eLife.22345
This record's URL: https://www.repository.cam.ac.uk/handle/1810/264674
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