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dc.contributor.authorHuang, Yen
dc.contributor.authorKim, JKen
dc.contributor.authorDo, DVen
dc.contributor.authorLee, Cen
dc.contributor.authorPenfold, Christopheren
dc.contributor.authorZylicz, Janen
dc.contributor.authorMarioni, Johnen
dc.contributor.authorHackett, Jamieen
dc.contributor.authorSurani, Azimen
dc.date.accessioned2017-06-07T14:01:48Z
dc.date.available2017-06-07T14:01:48Z
dc.date.issued2017-03-21en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264674
dc.description.abstractThe maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.
dc.description.sponsorshipThe work was funded by a studentship to YH from the James Baird Fund, University of Cambridge, by the DGIST Start-up Fund of the Ministry of Science, ICT and Future Planning to JKK, by a core grant from EMBL and CRUK to JCM, by a Wellcome Trust Senior Investigator Award to MAS, and by a core grant from the Wellcome Trust and Cancer Research UK to the Gurdon Institute.
dc.languageengen
dc.language.isoenen
dc.publishereLife Sciences Publications Ltd
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject2-cellen
dc.subjectMuERV-L/MERVLen
dc.subjectStellaen
dc.subjectchimeric transcriptsen
dc.subjectdevelopmental biologyen
dc.subjectmaternal-to-zygotic transitionen
dc.subjectmouseen
dc.subjectstem cellsen
dc.subjecttransposable elementsen
dc.titleStella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transitionen
dc.typeArticle
prism.numbere22345en
prism.publicationDate2017en
prism.publicationNameeLifeen
prism.volume6en
dc.identifier.doi10.17863/CAM.10313
dcterms.dateAccepted2017-03-09en
rioxxterms.versionofrecord10.7554/eLife.22345en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-03-21en
dc.contributor.orcidPenfold, Christopher [0000-0001-5823-4705]
dc.contributor.orcidZylicz, Jan [0000-0001-9622-5658]
dc.contributor.orcidMarioni, John [0000-0001-9092-0852]
dc.contributor.orcidHackett, Jamie [0000-0002-6237-3684]
dc.contributor.orcidSurani, Azim [0000-0002-8640-4318]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (096738/Z/11/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idCancer Research UK (A14492)
cam.orpheus.successThu Jan 30 12:53:47 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International